Current Microbiology

, Volume 34 , Issue 4 , pp 212 -215

First online:

Effect of Bacteria on Survival and Growth of Acanthamoeba castellanii

  • Xiaohui  WangAffiliated withGeorgia State University, Biology Department, P.O. Box 4010, Atlanta, GA 30303-4010, USA
  • , Donald G.  AhearnAffiliated withGeorgia State University, Biology Department, P.O. Box 4010, Atlanta, GA 30303-4010, USA

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract.

The growth and survival of Acanthamoeba castellanii in the presence of Gram-negative bacteria such as Pseudomonas aeruginosa, Escherichia coli, Serratia marcescens, and Stenotrophomonas maltophilia varied with the densities and species of bacteria. All species of bacteria suspended in a buffered saline at densities of 105 to 106/ml supported the growth and survival of 106/ml trophozoites of Acanthamoeba castellanii in a buffered saline solution. At densities of bacteria to amoebae of 100:1 or greater, growth and survival of A. castellanii were suppressed, particularly by P. aeruginosa. In an enrichment medium, the rapid growth of most co-inoculated bacteria inhibited the growth and survival of the amoeba.