Current Microbiology

, Volume 43, Issue 1, pp 51–56

An Efficient Method for Qualitative Screening of Phosphate-Solubilizing Bacteria

Authors

  • Sangeeta Mehta
    • Microbiology Group, National Botanical Research Institute, Rana Pratap Marg, P.B. No. 436, Lucknow 226 001, India
  • Chandra Shekhar Nautiyal
    • Microbiology Group, National Botanical Research Institute, Rana Pratap Marg, P.B. No. 436, Lucknow 226 001, India

DOI: 10.1007/s002840010259

Cite this article as:
Mehta, S. & Nautiyal, C. Curr Microbiol (2001) 43: 51. doi:10.1007/s002840010259

Abstract

An efficient protocol was developed for qualitative screening of phosphate-solubilizing bacteria, based upon visual observation. Our results indicate that, by using our formulation containing bromophenol blue, it is possible to quickly screen on a qualitative basis the phosphate-solubilizing bacteria. Qualitative analysis of the phosphate solubilized by various groups correlated well with grouping based upon quantitative analysis of bacteria isolated from soil, effect of carbon, nitrogen, salts, and phosphate solubilization-defective transposon mutants. However, unlike quantitative analysis methods that involve time-consuming biochemical procedures, the time for screening phosphate-solubilizing bacteria is significantly reduced by using our simple protocol. Therefore, it is envisaged that usage of this formulation based upon qualitative analysis will be salutary for the quick screening of phosphate-solubilizing bacteria. Our results indicate that the formulation can also be used as a quality control test for expeditiously screening the commercial bioinoculant preparations, based on phosphate solubilizers.

Copyright information

© Springer-Verlag New York Inc. 2001