Article

Current Microbiology

, Volume 42, Issue 3, pp 173-177

Enhanced Tolerance Against Salt-Stress and Freezing-Stress of Escherichia coli Cells Expressing Algal bbc1 Gene

  • Satoshi TanakaAffiliated withThe Kansai Electric Power Co., Technical Research Center, 11-20 Nakoji 3-Chome, Amagasaki, Hyogo 661-0974, Japan
  • , Kazunori IkedaAffiliated withKansai Environmental Engineering Center Co. Ltd, 3-5 Adzuchimachi 1-Chome Chuo-ku, Osaka, Osaka 541-0052, Japan
  • , Hitoshi MiyasakaAffiliated withThe Kansai Electric Power Co., Technical Research Center, 11-20 Nakoji 3-Chome, Amagasaki, Hyogo 661-0974, Japan

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

The expression of the eukaryotic bbc1 (breast basic conserved) gene (the bbc1 gene of the marine green alga Chlamydomonas sp. W-80 strain) enhanced the tolerance against salt-stress and freezing-stress in E. coli cells. The expression of the BBC1 protein in the E. coli cells carrying the algal bbc1 gene and that in the Chlamydomonas W-80 cells were examined by Western blotting analysis. The result suggests that the eukaryotic BBC1 protein expressed in the E. coli cells has a protective function against the cellular dehydration.