Enhanced Tolerance Against Salt-Stress and Freezing-Stress of Escherichia coli Cells Expressing Algal bbc1 Gene
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The expression of the eukaryotic bbc1 (breast basic conserved) gene (the bbc1 gene of the marine green alga Chlamydomonas sp. W-80 strain) enhanced the tolerance against salt-stress and freezing-stress in E. coli cells. The expression of the BBC1 protein in the E. coli cells carrying the algal bbc1 gene and that in the Chlamydomonas W-80 cells were examined by Western blotting analysis. The result suggests that the eukaryotic BBC1 protein expressed in the E. coli cells has a protective function against the cellular dehydration.
- Enhanced Tolerance Against Salt-Stress and Freezing-Stress of Escherichia coli Cells Expressing Algal bbc1 Gene
Volume 42, Issue 3 , pp 173-177
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- A1. The Kansai Electric Power Co., Technical Research Center, 11-20 Nakoji 3-Chome, Amagasaki, Hyogo 661-0974, Japan, JP
- A2. Kansai Environmental Engineering Center Co. Ltd, 3-5 Adzuchimachi 1-Chome Chuo-ku, Osaka, Osaka 541-0052, Japan, JP