Current Microbiology

, Volume 42, Issue 3, pp 173–177

Enhanced Tolerance Against Salt-Stress and Freezing-Stress of Escherichia coli Cells Expressing Algal bbc1 Gene

  • Satoshi Tanaka
  • Kazunori Ikeda
  • Hitoshi Miyasaka
Article

DOI: 10.1007/s002840010199

Cite this article as:
Tanaka, S., Ikeda, K. & Miyasaka, H. Curr Microbiol (2001) 42: 173. doi:10.1007/s002840010199

Abstract

The expression of the eukaryotic bbc1 (breast basic conserved) gene (the bbc1 gene of the marine green alga Chlamydomonas sp. W-80 strain) enhanced the tolerance against salt-stress and freezing-stress in E. coli cells. The expression of the BBC1 protein in the E. coli cells carrying the algal bbc1 gene and that in the Chlamydomonas W-80 cells were examined by Western blotting analysis. The result suggests that the eukaryotic BBC1 protein expressed in the E. coli cells has a protective function against the cellular dehydration.

Copyright information

© Springer-Verlag New York Inc. 2001

Authors and Affiliations

  • Satoshi Tanaka
    • 1
  • Kazunori Ikeda
    • 2
  • Hitoshi Miyasaka
    • 1
  1. 1.The Kansai Electric Power Co., Technical Research Center, 11-20 Nakoji 3-Chome, Amagasaki, Hyogo 661-0974, JapanJP
  2. 2.Kansai Environmental Engineering Center Co. Ltd, 3-5 Adzuchimachi 1-Chome Chuo-ku, Osaka, Osaka 541-0052, JapanJP