Current Microbiology

, Volume 67, Issue 6, pp 712–717

Attachment and Biofilm Forming Capabilities of Staphylococcus epidermidis Strains Isolated from Preterm Infants

  • Eva Hell
  • Christian G. Giske
  • Kjell Hultenby
  • Kristina Gemzell Danielsson
  • Giovanna Marchini
Article

DOI: 10.1007/s00284-013-0425-3

Cite this article as:
Hell, E., Giske, C.G., Hultenby, K. et al. Curr Microbiol (2013) 67: 712. doi:10.1007/s00284-013-0425-3

Abstract

Staphylococcus epidermidis, a human commensal, is an important opportunistic, biofilm-forming pathogen and the main cause of late onset sepsis in preterm infants, worldwide. In this study we describe the characteristics of S. epidermidis strains causing late onset (>72 h) bloodstream infection in preterm infants and skin isolates from healthy newborns. Attachment and biofilm formation capability were analyzed in microtiter plates and with transmission electron microscopy (TEM). Clonal relationship among strains was studied with pulsed-field gel electrophoresis. Antimicrobial susceptibility testing was performed, as well as the detection of biofilm-associated genes and of the invasiveness marker IS256 with polymerase chain reaction. Blood and skin isolates had similar attachment and biofilm-forming capabilities and biofilm formation was not related to the presence of specific genes. Filament-like membrane structures were seen by TEM early in the attachment close to the device surface, both in blood and skin strains. Nine of the ten blood isolates contained the IS256 and were also resistant to methicillin and gentamicin in contrast to skin strains. S. epidermidis strains causing bloodstream infection in preterm infants exhibit higher antibiotic resistance and are provided with an invasive genetic equipment compared to skin commensal strains. Adhesion capability to a device surface seems to involve bacterial membrane filaments.

Supplementary material

284_2013_425_MOESM1_ESM.docx (672 kb)
Supplementary material 1 (DOCX 671 kb)

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Eva Hell
    • 1
  • Christian G. Giske
    • 2
  • Kjell Hultenby
    • 3
  • Kristina Gemzell Danielsson
    • 1
  • Giovanna Marchini
    • 1
  1. 1.Department Women’s and Children’s Health, Karolinska University HospitalKarolinska InstitutetStockholmSweden
  2. 2.Department of Microbiology, Tumor and Cell Biology, Karolinska InstitutetKarolinska University HospitalStockholmSweden
  3. 3.Department of Laboratory MedicineKarolinska InstitutetStockholmSweden