Current Microbiology

, Volume 67, Issue 5, pp 580–589

Trichoderma reesei Histone Acetyltransferase Gcn5 Regulates Fungal Growth, Conidiation, and Cellulase Gene Expression

Authors

  • Qi Xin
    • State Key Laboratory of Microbial TechnologySchool of Life Science, Shandong University
  • Yajuan Gong
    • State Key Laboratory of Microbial TechnologySchool of Life Science, Shandong University
  • Xinxing Lv
    • State Key Laboratory of Microbial TechnologySchool of Life Science, Shandong University
  • Guanjun Chen
    • State Key Laboratory of Microbial TechnologySchool of Life Science, Shandong University
    • State Key Laboratory of Microbial TechnologySchool of Life Science, Shandong University
Article

DOI: 10.1007/s00284-013-0396-4

Cite this article as:
Xin, Q., Gong, Y., Lv, X. et al. Curr Microbiol (2013) 67: 580. doi:10.1007/s00284-013-0396-4

Abstract

Gcn5 is a well-established histone acetyltransferase involved in chromatin modification by catalyzing the acetylation of specific lysine residues within the N-terminal tails of the core histones. To assess the role of chromatin remodeling in the transcriptional response of cellulolytic Trichoderma reesei to the changes of environmental conditions, we identified the T. reesei ortholog of Saccharomyces cerevisiae Gcn5 by sequence alignment and functional analysis. Heterologous expression of TrGcn5 in S. cerevisiaegcn5Δ strain restored the growth defect under nutrient limitation as well as stresses. In contrast, mutant TrGcn5 with site-directed changes of residues critical for Gcn5 histone acetyltransferase activity could not complement the growth defect. The T. reeseigcn5Δ mutant strain displayed a strongly decreased growth rate and dramatic morphological changes including misshapen hyphal cells and abolished conidiation. Moreover, the induced expression of cellulase genes was severely impaired in the gcn5Δ T. reesei with acetylation of K9 and K14 of histone H3 in the cellulase gene promoter dramatically affected in the absence of TrGcn5. The results indicate that TrGcn5 plays a critical role in filamentous growth, morphogenesis, and transcriptional activation of specific genes including cellulase encoding genes.

Supplementary material

284_2013_396_MOESM1_ESM.doc (50 kb)
Supplementary material 1 (DOC 50 kb)
284_2013_396_MOESM2_ESM.doc (36 kb)
Supplementary material 2 (DOC 35 kb)

Copyright information

© Springer Science+Business Media New York 2013