Current Microbiology

, Volume 66, Issue 1, pp 72–78

Klebsiella michiganensis sp. nov., A New Bacterium Isolated from a Tooth Brush Holder

  • Ratul Saha
  • Christine E. Farrance
  • Bindhu Verghese
  • Sunhee Hong
  • Robert S. Donofrio
Article

DOI: 10.1007/s00284-012-0245-x

Cite this article as:
Saha, R., Farrance, C.E., Verghese, B. et al. Curr Microbiol (2013) 66: 72. doi:10.1007/s00284-012-0245-x

Abstract

Isolate W14T recovered from a household tooth brush holder was found to be gram-negative, a facultative anaerobic, non-motile, capsulated, and a non-endospore-forming straight rod. Based on phylogenetic analysis with 16S rRNA gene sequence, isolate W14T was affiliated to the genus Klebsiella. The closest phylogenetic relative was K. oxytoca with 99 % similarity in the 16S rRNA gene sequence. The major whole-cell fatty acids were C16:0 (31.23 %), C18:1ω6c/C18:1ω7c (21.10 %), and C16:1ω7c/C16:1ω6c (19.05 %). The sequence similarities of isolate W14T based on rpoB, gyrA, and gyrB were 97, 98, and 98 % with K. oxytoca, and 97, 93, and 90 % with K. mobilis (=Enterobacter aerogenes), respectively. The ribotyping pattern showed a 0.46 similarity with K. oxytoca ATCC 13182T and 0.24 with K. mobilis ATCC 13048T. The DNA G+C content of isolate W14T was 54.6 mol%. The DNA–DNA relatedness was 55.7 % with K. oxytoca ATCC 13182T. Using the identification technology of MALDI-TOF mass spectrometry, the top matches for this isolate were K. oxytoca ATCC 13182T (Match Factor Score 1.998) and K. mobilis (Score 1.797). On the basis of phenotypic, biochemical, chemotaxonomic, and molecular studies, isolate W14T could be differentiated from other members of the genus Klebsiella including K. mobilis. Therefore, it is proposed that isolate W14T (=ATCC BAA-2403T=DSM 25444T) should be classified as the type strain of a novel species of the genus Klebsiella, K. michiganensis sp. nov.

Supplementary material

284_2012_245_MOESM1_ESM.doc (42 kb)
Supplementary material 1 (DOC 42 kb)
284_2012_245_MOESM2_ESM.doc (980 kb)
Supplementary material 2 (DOC 979 kb)
284_2012_245_MOESM3_ESM.doc (77 kb)
Supplementary material 3 (DOC 77 kb)
284_2012_245_MOESM4_ESM.pdf (30 kb)
Supplementary material 4 (PDF 29 kb)

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Ratul Saha
    • 1
  • Christine E. Farrance
    • 2
  • Bindhu Verghese
    • 2
  • Sunhee Hong
    • 2
  • Robert S. Donofrio
    • 1
  1. 1.Microbiology DivisionNSF InternationalAnn ArborUSA
  2. 2.Accugenix Inc.NewarkUSA