Current Microbiology

, Volume 66, Issue 1, pp 56–60

Synergistic Effect and Antibiofilm Activity Between the Antimicrobial Peptide Coprisin and Conventional Antibiotics Against Opportunistic Bacteria

  • In-sok Hwang
  • Jae-Sam Hwang
  • Ji Hong Hwang
  • Hyemin Choi
  • Eunjung Lee
  • Yangmee Kim
  • Dong Gun Lee
Article

DOI: 10.1007/s00284-012-0239-8

Cite this article as:
Hwang, I., Hwang, JS., Hwang, J.H. et al. Curr Microbiol (2013) 66: 56. doi:10.1007/s00284-012-0239-8
  • 717 Downloads

Abstract

Coprisin is a 43-mer defensin-like peptide from the dung beetle, Copris tripartitus. In this study, we tested its minimum inhibitory concentration and performed combination assays to confirm the antibacterial susceptibility of coprisin and synergistic effects with antibiotics. The synergistic effects were evaluated by testing the effects of coprisin in combination with ampicillin, vancomycin, and chloramphenicol. The results showed that coprisin possessed antibacterial properties and had synergistic activities with the antibiotics. To understand the synergistic mechanism(s), we conducted hydroxyl radical assays. Coprisin alone and in combination with antibiotics generated hydroxyl radicals, which are highly reactive oxygen forms and the major property of bactericidal agents. Furthermore, the antibiofilm effect of coprisin alone and in combination with antibiotics was investigated. Biofilm formation is the source of many relentless and chronic bacterial infections. The results indicated that coprisin alone and in combination with antibiotics also had antibiofilm activity. Therefore, we conclude that coprisin has the potential to be used as a combinatorial therapeutic agent for the treatment of infectious diseases caused by bacteria.

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • In-sok Hwang
    • 1
  • Jae-Sam Hwang
    • 2
  • Ji Hong Hwang
    • 1
  • Hyemin Choi
    • 1
  • Eunjung Lee
    • 3
  • Yangmee Kim
    • 3
  • Dong Gun Lee
    • 1
  1. 1.School of Life Sciences and Biotechnology, College of Natural SciencesKyungpook National UniversityDaeguRepublic of Korea
  2. 2.National Academy of Agricultural ScienceRural Development AdministrationSuwonRepublic of Korea
  3. 3.Department of Bioscience and BiotechnologyKonkuk UniversitySeoulRepublic of Korea