Current Microbiology

, Volume 62, Issue 6, pp 1767–1773

Role of the all1549 (ana-rsh) Gene, A relA/spoT Homolog, of the Cyanobacterium Anabaena sp. PCC7120

  • Degang Ning
  • Yaru Qian
  • Xiaogang Miao
  • Chongwei Wen
Article

DOI: 10.1007/s00284-011-9926-0

Cite this article as:
Ning, D., Qian, Y., Miao, X. et al. Curr Microbiol (2011) 62: 1767. doi:10.1007/s00284-011-9926-0

Abstract

The role of a single relA/spoT homolog all1549 (designated hereafter as ana-rsh) of the cyanobacterium Anabaena sp. PCC7120 was investigated. The complementation test in Escherichiacoli showed that the protein encoded by ana-rsh possesses guanosine tetraphosphate (p)ppGpp-synthase/hydrolase activity. Under laboratory growth conditions, a low level of ppGpp was detected in Anabaena sp. PCC7120 and the loss of ana-rsh was lethal. Amino acid starvation induced ppGpp accumulation to an appropriate level, and nitrogen deficiency did not alter the ppGpp concentration in Anabaena cells. These data suggest that ana-rsh is required for cell viability under normal growth conditions and involved in the (p)ppGpp-related stringent response to amino acid deprivation, but not related to heterocyst formation and nitrogen fixation of Anabaena sp. PCC7120.

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Degang Ning
    • 1
  • Yaru Qian
    • 2
  • Xiaogang Miao
    • 1
  • Chongwei Wen
    • 3
  1. 1.Department of Environment Sciences, College of EnvironmentJiangsu UniversityZhenjiangChina
  2. 2.Institute of Life SciencesJiangsu UniversityZhenjiangChina
  3. 3.Department of Pharmacy, College of PharmacyJiangsu UniversityZhenjiangChina