Article

Current Microbiology

, Volume 62, Issue 3, pp 758-763

First online:

Characterization of Novel Alleles of Toxin Co-Regulated Pilus A Gene (tcpA) from Environmental Isolates of Vibrio cholerae

  • Praveen KumarAffiliated withDepartment of Molecular Microbiology, Cholera and Environmental Microbiology Laboratory, Rajiv Gandhi Centre for Biotechnology
  • , Anuja ThulaseedharanAffiliated withDepartment of Molecular Microbiology, Cholera and Environmental Microbiology Laboratory, Rajiv Gandhi Centre for Biotechnology
  • , Gautam ChowdhuryAffiliated withNational Institute of Cholera and Enteric Diseases
  • , Thandavarayan RamamurthyAffiliated withNational Institute of Cholera and Enteric Diseases
  • , Sabu ThomasAffiliated withDepartment of Molecular Microbiology, Cholera and Environmental Microbiology Laboratory, Rajiv Gandhi Centre for Biotechnology Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Vibrio cholerae is causative agent of life threatening diarrheal disease, cholera. The toxin co-regulated pilus (TCP) is a critical colonization factor of V. cholerae and it also serves as receptor for CTXФ. In this study, we describe nucleotide sequence of four novel alleles of tcpA gene from toxigenic and non-toxigenic V. cholerae isolated from environmental sources. The phylogenetic analysis of tcpA revealed that it is related to tcpA of newly emerged O1 strain and unrelated to tcpA of wild type (classical and El Tor strains). All strains showed variant tcpA and also harbored intact Vibrio Pathogenicity Island (VPI). The expression of all variant alleles was demonstrated by RT-PCR.