Current Microbiology

, Volume 62, Issue 3, pp 758–763

Characterization of Novel Alleles of Toxin Co-Regulated Pilus A Gene (tcpA) from Environmental Isolates of Vibrio cholerae

  • Praveen Kumar
  • Anuja Thulaseedharan
  • Gautam Chowdhury
  • Thandavarayan Ramamurthy
  • Sabu Thomas
Article

DOI: 10.1007/s00284-010-9774-3

Cite this article as:
Kumar, P., Thulaseedharan, A., Chowdhury, G. et al. Curr Microbiol (2011) 62: 758. doi:10.1007/s00284-010-9774-3
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Abstract

Vibrio cholerae is causative agent of life threatening diarrheal disease, cholera. The toxin co-regulated pilus (TCP) is a critical colonization factor of V. cholerae and it also serves as receptor for CTXФ. In this study, we describe nucleotide sequence of four novel alleles of tcpA gene from toxigenic and non-toxigenic V. cholerae isolated from environmental sources. The phylogenetic analysis of tcpA revealed that it is related to tcpA of newly emerged O1 strain and unrelated to tcpA of wild type (classical and El Tor strains). All strains showed variant tcpA and also harbored intact Vibrio Pathogenicity Island (VPI). The expression of all variant alleles was demonstrated by RT-PCR.

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Praveen Kumar
    • 1
  • Anuja Thulaseedharan
    • 1
  • Gautam Chowdhury
    • 2
  • Thandavarayan Ramamurthy
    • 2
  • Sabu Thomas
    • 1
  1. 1.Department of Molecular Microbiology, Cholera and Environmental Microbiology LaboratoryRajiv Gandhi Centre for BiotechnologyTrivandrumIndia
  2. 2.National Institute of Cholera and Enteric DiseasesKolkataIndia

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