Current Microbiology

, Volume 62, Issue 2, pp 486–491

Desulfovibrio legallis sp. nov.: A Moderately Halophilic, Sulfate-Reducing Bacterium Isolated from a Wastewater Digestor in Tunisia

  • Olfa Ben Dhia Thabet
  • Terres Wafa
  • Khelifi Eltaief
  • Jean-Luc Cayol
  • Moktar Hamdi
  • Guy Fauque
  • Marie-Laure Fardeau
Article

DOI: 10.1007/s00284-010-9733-z

Cite this article as:
Thabet, O.B.D., Wafa, T., Eltaief, K. et al. Curr Microbiol (2011) 62: 486. doi:10.1007/s00284-010-9733-z

Abstract

A new moderately halophilic sulfate-reducing bacterium (strain H1T) was enriched and isolated from a wastewater digestor in Tunisia. Cells were curved, motile rods (2–3 x 0.5 μm). Strain H1T grew at temperatures between 22 and 43°C (optimum 35°C), and at pH between 5.0 and 9.2 (optimum 7.3–7.5). Strain H1T required salt for growth (1–45 g of NaCl/l), with an optimum at 20–30 g/l. Sulfate, sulfite, thiosulfate, and elemental sulfur were used as terminal electron acceptors but not nitrate and nitrite. Strain H1T utilized lactate, pyruvate, succinate, fumarate, ethanol, and hydrogen (in the presence of acetate and CO2) as electron donors in the presence of sulfate as electron acceptor. The main end-products from lactate oxidation were acetate with H2 and CO2. The G + C content of the genomic DNA was 55%. The predominant fatty acids of strain H1T were C15:0 iso (38.8%), C16:0 (19%), and C14:0 iso 3OH (12.2%), and menaquinone MK-6 was the major respiratory quinone. Phylogenetic analysis of the small-subunit (SSU) ribosomal RNA (rRNA) gene sequence indicated that strain H1T was affiliated to the genus Desulfovibrio. On the basis of SSU rRNA gene sequence comparisons and physiological characteristics, strain H1T is proposed to be assigned to a novel species of sulfate reducers of the genus Desulfovibrio, Desulfovibrio legallis sp. nov. (= DSM 19129T = CCUG 54389T).

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Olfa Ben Dhia Thabet
    • 1
  • Terres Wafa
    • 1
  • Khelifi Eltaief
    • 1
  • Jean-Luc Cayol
    • 2
  • Moktar Hamdi
    • 1
  • Guy Fauque
    • 2
  • Marie-Laure Fardeau
    • 2
  1. 1.INSAT, Laboratoire d’Ecologie et de Technologie Microbienne, Centre Urbain NordTunis CedexTunisia
  2. 2.Laboratoire de Microbiologie IRD, UMR 180Universités de Provence et de la Méditerranée, ESIL, Case 925Marseille Cedex 9France