Current Microbiology

, Volume 60, Issue 6, pp 454–460

Inhibition of Streptococcus pyogenes Biofilm Formation by Coral-Associated Actinomycetes


  • Paramasivam Nithyanand
    • Department of BiotechnologyAlagappa University
  • Ramalingam Thenmozhi
    • Department of BiotechnologyAlagappa University
  • Janarthanam Rathna
    • Department of BiotechnologyAlagappa University
    • Department of BiotechnologyAlagappa University

DOI: 10.1007/s00284-009-9564-y

Cite this article as:
Nithyanand, P., Thenmozhi, R., Rathna, J. et al. Curr Microbiol (2010) 60: 454. doi:10.1007/s00284-009-9564-y


Streptococcus pyogenes biofilms tend to exhibit significant tolerance to antimicrobials during infections. We screened coral-associated actinomycetes (CAA) for antibiofilm activity against different biofilm forming M serotype of Streptococcus pyogenes. Actinomycetes isolated from the mucus of the coral Acropora digitifera were screened for antibiofilm activity against S. pyogenes biofilms wherein several isolates clearly demonstrated antibiofilm activity. The biofilm inhibitory concentrations (BICs) and the sub-BICs (1/2 and 1/4 BIC) of the extracts significantly prevented biofilm formation up to 60–80%. The extract of Streptomyces akiyoshinensis (A3) displayed efficient antibiofilm activity against all the biofilm forming M serotypes. All the five extracts efficiently reduced the cell surface hydrophobicity (a crucial factor for biofilm formation in S. pyogenes) of three M types and thus may inhibit biofilm formation. CAA represent an interesting source of marine invertebrates-derived antibiofilm agents in the development of new strategies to combat Streptococcal biofilms.

Supplementary material

284_2009_9564_MOESM1_ESM.docx (17 kb)
Supplementary material 1 (DOCX 17 kb)

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© Springer Science+Business Media, LLC 2009