Current Microbiology

, Volume 57, Issue 2, pp 121–126

Characterization of a Highly Active Promoter, PBbgpd, in Beauveria bassiana

  • Xing-gang Liao
  • Wei-guo Fang
  • Yong-jun Zhang
  • Yan-hua Fan
  • Xing-wei Wu
  • Qun Zhou
  • Yan Pei
Article

DOI: 10.1007/s00284-008-9163-3

Cite this article as:
Liao, X., Fang, W., Zhang, Y. et al. Curr Microbiol (2008) 57: 121. doi:10.1007/s00284-008-9163-3

Abstract

The promoter of glyceraldehyde-3-phosphate dehydrogenase (gpd) gene from Aspergillus nidulans (PgpdA) is widely used to direct expression of target genes constitutively in fungi. However, in some species, a heterogeneous promoter is found to be of low efficiency. To obtain a high-efficiency promoter for transformation of Beauveria bassiana, an entomopathogenic fungus widely used as an mycoinsecticide, a glyceraldehyde-3-phosphate dehydrogenase gene (Bbgpd) promoter, was cloned and characterized. Four deletion constructs (−2118, −1153, −726, and −354) of the 5′-upstream sequence of Bbgpd linked to a bar::gus fusion gene (phosphinothricin-resistance::β-glucuronidase fused gene), which were used as selected marker gene and report gene, respectively, were generated. GUS activities of transgenic strains harboring −726, −1153, and −2118 deletion constructs were much stronger than that of the promoter of Aspergillus nidulansgpdA (PgpdA), with a twofold to threefold increase over that in the PgpdA construct. The −726 fragment was necessary to direct GUS expression in B. bassiana. No −354 transgenic progenies were obtained, possibly because it failed to initiate the transcription of bar::gus fusion gene. A remarkable increase of GUS activity was found between the −1153 and −726 constructs, indicating that some active transcriptional elements were located in this region. With a high expression level and relatively short sequence, PBbgpd can be used to drive target genes in B. bassiana transgenic research.

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Xing-gang Liao
    • 1
  • Wei-guo Fang
    • 1
  • Yong-jun Zhang
    • 1
  • Yan-hua Fan
    • 1
  • Xing-wei Wu
    • 1
  • Qun Zhou
    • 1
  • Yan Pei
    • 1
  1. 1.Biotechnology Research Center, Key Laboratory of Biotechnology & Crop Quality Improvement, Ministry of AgricultureSouthwest UniversityChongqingPeople’s Republic of China