Current Microbiology

, Volume 51, Issue 2, pp 91–94

Characterization and Transcription of the Genes Involved in Butyrate Production in Butyrivibrio fibrisolvens TypeI and II Strains

  • Narito Asanuma
  • Masaya Ishiwata
  • Takahiro Yoshii
  • Masahiro Kikuchi
  • Youichi Nishina
  • Tsuneo Hino
Article

DOI: 10.1007/s00284-005-4477-x

Cite this article as:
Asanuma, N., Ishiwata, M., Yoshii, T. et al. Curr Microbiol (2005) 51: 91. doi:10.1007/s00284-005-4477-x

Abstract

The genes in Butyrivibrio fibrisolvens that encode the enzymes involved in butyrate production were sequenced. In a type I strain (ATCC 19171T), the genes coding for the enzymes that catalyze the conversion from acetyl-CoA to butyryl-CoA, thl (thiolase), crt (crotonase), hbd (β-hydroxybutyryl-CoA dehydrogenase), bcd (butyryl-CoA dehydrogenase), etfB (electron transfer flavoprotein [ETF]-β), and etfA (ETF-α), were found to be clustered and arranged in this order. A type II strain (ATCC 51255) had the same clustered genes with the same arrangement, except that crt was not present in the clustered genes. The deduced amino acid sequences of these enzymes did not greatly differ between the two strains, and even between B. fibrisolvens and clostridia. Amino acid identity appeared to be higher within the same type than between types I and II. The clustered genes were shown to be cotranscribed, and constitutively transcribed without being affected significantly by culture conditions.

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Narito Asanuma
    • 1
  • Masaya Ishiwata
    • 1
  • Takahiro Yoshii
    • 1
  • Masahiro Kikuchi
    • 1
  • Youichi Nishina
    • 1
  • Tsuneo Hino
    • 1
  1. 1.Department of Life Science, College of AgricultureMeiji University, HigashimitaTama-kuJapan

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