Current Microbiology

, Volume 54, Issue 2, pp 85–90

Bactericidal Monoclonal Antibody Against Moraxella catarrhalis Lipooligosaccharide Cross-Reacts with Haemophilus Spp.

  • Raina T. Gergova
  • Ianko D. Iankov
  • Iana H. Haralambieva
  • Ivan G. Mitov
Article

DOI: 10.1007/s00284-005-0463-6

Cite this article as:
Gergova, R.T., Iankov, I.D., Haralambieva, I.H. et al. Curr Microbiol (2007) 54: 85. doi:10.1007/s00284-005-0463-6

Abstract

Monoclonal antibodies (MAbs) against lipooligosaccharide (LOS) determinants after immunization of BALB/c mice with heat inactivated Moraxella catarrhalis serotype A were generated. MAb 219A9 was specific for a common epitope of A, B, and C M. catarrhalis serotypes in ELISA and immunofluorescent test (IFT). In both tests it also cross-reacted with whole bacteria and LPS antigens isolated from non-typeable H. influenzae and H. parainfluenzae strains. IgM antibody clone 219A9 possessed a strong bactericidal effect against the three serotypes in the presence of complement. Our results demonstrate that antibodies directed to a single LOS epitope common for A, B, and C serotype could be highly protective. This suggests that the common determinants are very promising in the development of LOS-based vaccine against M. catarrhalis. The cross-reactions of MAb 219A9 with Haemophilus spp. also show that immunization could result in immune response to epitopes conserved in other important respiratory pathogens.

Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Raina T. Gergova
    • 1
  • Ianko D. Iankov
    • 1
  • Iana H. Haralambieva
    • 1
  • Ivan G. Mitov
    • 1
  1. 1.Department of MicrobiologyMedical UniversitySofiaBulgaria