Current Microbiology

, Volume 52, Issue 3, pp 186–190

Colonization of Maize and Rice Plants by Strain Bacillus megaterium C4


DOI: 10.1007/s00284-005-0162-3

Cite this article as:
Liu, X., Zhao, H. & Chen, S. Curr Microbiol (2006) 52: 186. doi:10.1007/s00284-005-0162-3


Bacillus megaterium C4, a nitrogen-fixing bacterium, was marked with the gfp gene. Maize and rice seedlings were inoculated with the, GFP-labeled B. megaterium C4 and then grown in gnotobiotic condition. Observation by confocal laser scanning microscope showed that the GFP-labeled bacterial cells infected the maize roots through the cracks formed at the lateral root junctions and then penetrated into cortex, xylem, and pith, and that the bacteria migrated slowly from roots to stems and leaves. The bacteria were mainly located in the intercellular spaces, although a few bacterial cells were also present within the xylem vessels, root hair cells, epidermis, cortical parenchyma, and pith cells. In addition, microscopic observation also revealed clearly that the root tip in the zone of elongation and differentiation and the junction between the primary and the lateral roots were the two sites for the bacteria entry into rice root. Therefore, we conclude that this Gram-positive nitrogen-fixer has a colonization pattern similar to those of many Gram-negative diazotrophs, such as Azospirillun brasilense Yu62 and Azoarcus sp. As far as we know, this is the first detailed report of the colonization pattern for Gram-positive diazotrophic Bacillus.

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.State Key Laboratory for Agrobiotechnology and College of Biological SciencesChina Agricultural UniversityP. R. China
  2. 2.Laboratory for Agro-Microbial Resource and Application of Ministry of AgricultureP. R. China
  3. 3.Chemical and Biological SchoolShenYang Normal UniversityP. R. China

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