Current Microbiology

, Volume 51, Issue 5, pp 317–321

Recovery of Partial 16S rDNA Sequences Suggests the Presence of Crenarchaeota in the Human Digestive Ecosystem

  • Françoise Rieu-Lesme
  • Céline Delbès
  • Lauriane Sollelis
Article

DOI: 10.1007/s00284-005-0036-8

Cite this article as:
Rieu-Lesme, F., Delbès, C. & Sollelis, L. Curr Microbiol (2005) 51: 317. doi:10.1007/s00284-005-0036-8

Abstract

Human feces collected from 10 healthy teenagers was analyzed for the presence of Crenarchaeota. After a first polymerase chain reaction (PCR) with Archaea-specific primers, a nested real-time PCR was performed using Crenarchaeota-specific primers. Real-time Crenarchaeotal PCR products detected from four subjects were cloned and the sequencing revealed that most of the partial 16S rRNA gene sequences were highly similar (≥97% homology) to sequences affiliated to the Sulfolobus group of the Crenarchaeota phylum. Our findings suggest for the first time that Crenarchaeota might be present in the microbiota of the human digestive ecosystem in which this phylum has never been found yet.

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Françoise Rieu-Lesme
    • 1
  • Céline Delbès
    • 2
  • Lauriane Sollelis
    • 1
  1. 1.Unité de MicrobiologieInstitut National de la Recherche Agronomique (I.N.R.A.)France
  2. 2.Unité de Recherches FromagèresI.N.R.A.France