Article

Current Microbiology

, Volume 49, Issue 5, pp 372-375

First online:

Indirect Immunofluorescence Microscopy for Direct Detection of Xylella fastidiosa in Xylem Sap

  • Dulce CarbajalAffiliated withDepartment of Natural Sciences, University of Houston-Downtown
  • , Kevin A. MoranoAffiliated withDepartment of Microbiology and Molecular Genetics, University of Texas Medical School
  • , Lisa D. MoranoAffiliated withDepartment of Natural Sciences, University of Houston-Downtown Email author 

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Abstract

The plant pathogen Xylella fastidiosa is the causative agent of a number of diseases of economically important crops, including Pierce’s disease that affects grapevines. Using a commercially available antibody specific for X. fastidiosa, we have established a protocol for microscopic identification of the bacterium by indirect immunofluorescence. This antibody clearly labels an uncharacterized antigen concentrated at a single pole of X. fastidiosa cells, but does not react with a non-Xylella control. This technique was also performed successfully on xylem exudates from several different plant genera and correlated well with standard enzyme-linked immunosorbent assay tests. These results establish a novel method for in situ assessment of X. fastidiosa infection from host plants.