Current Microbiology

, Volume 49, Issue 5, pp 372–375

Indirect Immunofluorescence Microscopy for Direct Detection of Xylella fastidiosa in Xylem Sap

  • Dulce Carbajal
  • Kevin A. Morano
  • Lisa D. Morano
Article

DOI: 10.1007/s00284-004-4369-5

Cite this article as:
Carbajal, D., Morano, K.A. & Morano, L.D. Current Microbiology (2004) 49: 372. doi:10.1007/s00284-004-4369-5

Abstract

The plant pathogen Xylella fastidiosa is the causative agent of a number of diseases of economically important crops, including Pierce’s disease that affects grapevines. Using a commercially available antibody specific for X. fastidiosa, we have established a protocol for microscopic identification of the bacterium by indirect immunofluorescence. This antibody clearly labels an uncharacterized antigen concentrated at a single pole of X. fastidiosa cells, but does not react with a non-Xylella control. This technique was also performed successfully on xylem exudates from several different plant genera and correlated well with standard enzyme-linked immunosorbent assay tests. These results establish a novel method for in situ assessment of X. fastidiosa infection from host plants.

Copyright information

© Springer Science+Business Media, Inc. 2004

Authors and Affiliations

  • Dulce Carbajal
    • 1
  • Kevin A. Morano
    • 2
  • Lisa D. Morano
    • 1
  1. 1.Department of Natural SciencesUniversity of Houston-DowntownHoustonUSA
  2. 2.Department of Microbiology and Molecular GeneticsUniversity of Texas Medical SchoolHoustonUSA