Current Microbiology

, Volume 49, Issue 1, pp 10–12

Wolbachia Replication and Host Cell Division in Aedes albopictus

  • Toon Ruang-areerate
  • Pattamaporn Kittayapong
  • Elizabeth A. McGraw
  • Vitsut Baimai
  • Scott L. O’Neill
Article

DOI: 10.1007/s00284-003-4245-8

Cite this article as:
Ruang-areerate, ., Kittayapong, ., McGraw, . et al. Curr Microbiol (2004) 49: 10. doi:10.1007/s00284-003-4245-8

Abstract

Wolbachia pipientis is an obligate intracellular endosymbiont of a range of arthropod species. The microbe is best known for its manipulations of host reproduction that include inducing cytoplasmic incompatibility, parthenogenesis, feminization, and male-killing. Like other vertically transmitted intracellular symbionts, Wolbachia’s replication rate must not outpace that of its host cells if it is to remain benign. The mosquito Aedes albopictus is naturally infected both singly and doubly with different strains of Wolbachia pipientis. During diapause in mosquito eggs, no host cell division is believed to occur. Further development is triggered only by subsequent exposure of the egg to water. This study uses diapause in Wolbachia-infected Aedes albopictus eggs to determine whether symbiont replication slows or stops when host cell division ceases or whether it continues at a low but constant rate. We have shown that Wolbachia densities in eggs are greatest during embryonation and then decline throughout diapause, suggesting that Wolbachia replication is dependent on host cell replication.

Copyright information

© Springer-Verlag New York Inc. 2004

Authors and Affiliations

  • Toon Ruang-areerate
    • 2
  • Pattamaporn Kittayapong
    • 1
  • Elizabeth A. McGraw
    • 3
  • Vitsut Baimai
    • 1
  • Scott L. O’Neill
    • 3
  1. 1.Center for Vectors and Vector-Borne Diseases and Department of Biology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400Thailand
  2. 2.Section of Vector Biology, Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, CT 06520United States
  3. 3.Department of Zoology & Entomology, School of Life Sciences, University of Queensland, St. Lucia, Queensland 4072Australia

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