Current Microbiology

, Volume 44, Issue 4, pp 286–290

Optimization of Alkaline Protease Production from Bacillus sp. by Response Surface Methodology

  • Sumant Puri
  • Qasim Khalil Beg
  • Rani Gupta

DOI: 10.1007/s00284-001-0006-8

Cite this article as:
Puri, S., Beg, Q. & Gupta, R. Curr Microbiol (2002) 44: 286. doi:10.1007/s00284-001-0006-8

Abstract

High yields (1939 U/ml) of an alkaline protease were obtained in batch fermentation of a Bacillus sp. using a response surface methodology. The interaction of four variables, viz., starch, peptone, incubation time, and inoculum density, suggested inoculum density to be an insignificant variable. However, incubation time had a profound effect on protease yields at all the concentrations of carbon and nitrogen used. The response surface raised and flattened with increase in time of incubation, and maximum protease production up to 1939 U/ml was obtained after 96 h of incubation. The model equation obtained was validated experimentally at maximum starch (15 mg/ml) and peptone (7.5 mg/ml) concentration with increased incubation time up to 144 h in the presence of minimum inoculum density (1%). An overall 2.6-fold increase in protease production was obtained as compared with mean observed response (750 U/ml) at zero level of all variables.

Copyright information

© Springer-Verlag New York Inc. 2002

Authors and Affiliations

  • Sumant Puri
    • 1
  • Qasim Khalil Beg
    • 1
  • Rani Gupta
    • 1
  1. 1.Department of Microbiology, University of Delhi South Campus, Benito Juarez Marg, New Delhi 110 021, IndiaIN