Cancer Chemotherapy and Pharmacology

, Volume 46, Issue 6, pp 467–476

Effects of gemcitabine on cell proliferation and apoptosis in non-small-cell lung cancer (NSCLC) cell lines

  • E. Pace
  • M. Melis
  • L. Siena
  • F. Bucchieri
  • A. M. Vignola
  • M. Profita
  • M. Gjomarkaj
  • G. Bonsignore
ORIGINAL ARTICLE

DOI: 10.1007/s002800000183

Cite this article as:
Pace, E., Melis, M., Siena, L. et al. Cancer Chemother Pharmacol (2000) 46: 467. doi:10.1007/s002800000183

Abstract

We evaluated the antiproliferative and the proapoptotic ability of gemcitabine in three non-small-cell lung cancer (NSCLC) cell lines. NCI-H292 (mucoepidermoid carcinoma), NCI-CorL23 (large-cell carcinoma) and NCI-Colo699 (adenocarcinoma) cells were cultured with and without 0.5, 0.05 and 0.005 μM gemcitabine for 24, 48 and 72 h, respectively. Gemcitabine exerted a stronger and earlier antiproliferative and proapoptotic effect on H292 cells than on CorL23 or Colo699 cells. Fas receptor expression was increased in all three cell lines and was higher in Colo699 than in CorL23 cells. The incubation of NSCLC with anti-Fas agonistic monoclonal antibody (CH11) induced cell apoptosis in H292 cells, demonstrating that the Fas receptor was functionally active. Finally, gemcitabine and CH-11 exerted a synergistic effect on cell apoptosis in H292 cells. This study demonstrates that gemcitabine induces apoptosis in NSCLC and that this effect might be exerted by modulating functionally active Fas expression, and these effects of gemcitabine were stronger in H292 cells than in either CorL23 or Colo699 cells.

Key words GemcitabineNon-small-cell lung cancerNSCLCApoptosis

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • E. Pace
    • 1
  • M. Melis
    • 1
  • L. Siena
    • 1
  • F. Bucchieri
    • 1
  • A. M. Vignola
    • 1
  • M. Profita
    • 1
  • M. Gjomarkaj
    • 1
  • G. Bonsignore
    • 1
  1. 1.Italian National Research Council, Institute of Respiratory Pathophysiology, Via Trabucco 180, 90146 Palermo, Italy e-mail: pace@ifr.pa.cnr.it Tel.: +39-091-6882879; Fax: +39-091-6882165IT