Cancer Chemotherapy and Pharmacology

, Volume 65, Issue 5, pp 825–831

Epidermal growth factor receptor (EGFR) mRNA levels and protein expression levels in primary colorectal cancer and corresponding liver metastases

Authors

  • Hidekazu Kuramochi
    • Department of Gastroenterological SurgeryTokyo Women’s Medical University
    • Department of Biochemistry and Molecular BiologyUniversity of Southern California
    • Department of Gastroenterological SurgeryTokyo Women’s Medical University
  • Go Nakajima
    • Department of Gastroenterological SurgeryTokyo Women’s Medical University
  • Hirotaka Kamikozuru
    • Department of Gastroenterological SurgeryTokyo Women’s Medical University
  • Masakazu Yamamoto
    • Department of Gastroenterological SurgeryTokyo Women’s Medical University
  • Kathleen D. Danenberg
    • Response Genetics Inc
  • Peter V. Danenberg
    • Department of Biochemistry and Molecular BiologyUniversity of Southern California
Original Article

DOI: 10.1007/s00280-009-1087-5

Cite this article as:
Kuramochi, H., Hayashi, K., Nakajima, G. et al. Cancer Chemother Pharmacol (2010) 65: 825. doi:10.1007/s00280-009-1087-5

Abstract

Purpose

High expression levels of EGFR mRNA are reported to be associated with a higher response probability in epidermal growth factor receptor (EGFR) targeted drugs. Our aim was to determine how EGFR gene expression levels in primary colorectal cancer (CRC) were related to those in liver metastases.

Methods

31 pairs of primary CRC and corresponding liver metastases were analyzed. Gene expression level was measured using real-time RT-PCR.

Results

No significant difference was observed between median mRNA expression levels of EGFR in primary cancer and those in corresponding liver metastases (P = 0.99). When matched tissue sets were compared on an individual basis, there was a significant correlation for EGFR mRNA expression between primary cancer and corresponding liver metastases (rs = 0.78, P < 0.0001).

Conclusions

A good prediction of EGFR mRNA levels in liver metastases can be obtained by measuring those in the primary CRC.

Keywords

EGFRmRNALiver metastasesColorectal cancerGene expression

Copyright information

© Springer-Verlag 2009