Cancer Chemotherapy and Pharmacology

, Volume 64, Issue 1, pp 79–86

Evaluation of mRNA by Q-RTPCR and protein expression by AQUA of the M2 subunit of ribonucleotide reductase (RRM2) in human tumors

  • Jill Kolesar
  • Wei Huang
  • Jens Eickhoff
  • Kristine Hahn
  • Dona Alberti
  • Steven Attia
  • William Schelman
  • Kyle Holen
  • Anne Traynor
  • Percy Ivy
  • George Wilding
Original Article

DOI: 10.1007/s00280-008-0845-0

Cite this article as:
Kolesar, J., Huang, W., Eickhoff, J. et al. Cancer Chemother Pharmacol (2009) 64: 79. doi:10.1007/s00280-008-0845-0

Abstract

Purpose

The purpose of this study was to evaluate baseline RRM2 protein and gene expression in tumors of patients receiving 3-AP.

Methods

Tumor blocks from patients enrolled in phase I and II clinical studies using 3-AP, were evaluated for RRM2 gene and protein expression by quantitative real time polymerase chain reaction (Q-RTPCR) and automated quantitative analysis (AQUA).

Results

Esophageal and gastric cancers overexpressed RRM2 protein when compared to prostate cancer (Z-score, 0.68 ± 0.94 SD, vs 0.41 ± 0.84 SD, respectively; p = 0.04). Esophageal and gastric cancers also overexpressed RRM2 mRNA when compared to prostate cancer (relative gene expression 2.56 ± 1.49 SD, vs 0.29 ± 0.20 SD, respectively; = 0.02). Protein and gene expression were moderately associated (Spearman’s rank correlation = 0.30; p = 0.12).

Conclusion

RRM2 gene and protein expression varies by tumor type.

Keywords

Triapine®3-Aminopyridine-2-carboxaldehyde thiosemicarbazoneRibonucleotide reductaseAutomated quantitative immunohistochemistry (AQUA)Quantitative real time PCR

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Jill Kolesar
    • 1
    • 2
  • Wei Huang
    • 1
    • 3
  • Jens Eickhoff
    • 1
    • 4
  • Kristine Hahn
    • 1
    • 5
  • Dona Alberti
    • 1
    • 5
  • Steven Attia
    • 1
    • 5
  • William Schelman
    • 1
    • 5
  • Kyle Holen
    • 1
    • 5
  • Anne Traynor
    • 1
    • 5
  • Percy Ivy
    • 6
  • George Wilding
    • 1
    • 5
  1. 1.University of Wisconsin Paul P. Carbone Comprehensive Cancer CenterMadisonUSA
  2. 2.School of PharmacyUniversity of WisconsinMadisonUSA
  3. 3.Department of PathologyUniversity of Wisconsin School of Medicine and Public HealthMadisonUSA
  4. 4.Department of BiostatisticsUniversity of Wisconsin School of Medicine and Public HealthMadisonUSA
  5. 5.Department of MedicineUniversity of Wisconsin School of Medicine and Public HealthMadisonUSA
  6. 6.Cancer Therapy and Evaluation ProgramNational Cancer InstituteBethesdaUSA