Cancer Chemotherapy and Pharmacology

, Volume 60, Issue 1, pp 103–111

The apoptotic effect of cordycepin on human OEC-M1 oral cancer cell line

Authors

  • Wei-Ciao Wu
    • Department of Medicine, College of MedicineNational Cheng Kung University
  • Jenn-Ren Hsiao
    • Department of Otolaryngology, College of MedicineNational Cheng Kung University
  • Yu-Yan Lian
    • Department of Cell Biology and Anatomy, College of MedicineNational Cheng Kung University
  • Chun-Yu Lin
    • Department of Cell Biology and Anatomy, College of MedicineNational Cheng Kung University
    • Department of Cell Biology and Anatomy, College of MedicineNational Cheng Kung University
Original Article

DOI: 10.1007/s00280-006-0354-y

Cite this article as:
Wu, W., Hsiao, J., Lian, Y. et al. Cancer Chemother Pharmacol (2007) 60: 103. doi:10.1007/s00280-006-0354-y

Abstract

Cordycepin (3′-deoxyadenosine), a pure compound of Cordyceps sinensis, has been illustrated with anti-tumor effects. In the present study, the apoptotic effect of cordycepin on OEC-M1, a human oral squamous cancer cell line, was investigated by morphological observations, cell viability assay, annexin V-FITC analysis and flow cytometry methods. Results demonstrated that the number of rounded-up cell increased as treatment duration of cordycepin (100 μM) increased from 3 to 48 h, and the plasma membrane blebbing could be observed after 12 h treatment. In cell viability assay, cell surviving rate significantly decreased as the dosage and duration of cordycepin treatment increased (< 0.05). Moreover, phosphatidylserine flipping on cell membrane could be detected with 3, 6 and 12 h cordycepin treatment, which indicated an early apoptotic phenomenon. Furthermore, cell cycle studies illustrated that the percentage of G1 phase cell declined as the dosages of cordycepin increased (10 μM to 5 mM), while the percentages of G2M and subG1 phase cell increased (< 0.05) in 12, 24 and 48 h cordycepin treatment. These results further confirmed the apoptotic event. In conclusion, cordycepin significantly induced cell apoptotsis in OEC-M1 human oral squamous cancer cells.

Keywords

CordycepinApoptosisOEC-M1Oral cancerCell cycleCell viability

Copyright information

© Springer-Verlag 2006