Annals of Hematology

, Volume 78, Issue 3, pp 117–123

G-CSF versus GM-CSF for stimulation of peripheral blood progenitor cells (PBPC) and leukocytes in healthy volunteers: comparison of efficacy and tolerability

  • G. Fischmeister
  • M. Kurz
  • O. A. Haas
  • M. Micksche
  • P. Buchinger
  • D. Printz
  • G. Ressmann
  • T. Stroebel
  • C. Peters
  • G. Fritsch
  • H. Gadner
ORIGINAL ARTICLE

DOI: 10.1007/s002770050487

Cite this article as:
Fischmeister, G., Kurz, M., Haas, O. et al. Ann Hematol (1999) 78: 117. doi:10.1007/s002770050487

Abstract

 This study compared two recombinant human (rh) hematopoietic growth factors in healthy volunteers for stem cell stimulation. Granulocyte colony-stimulating factor (G-CSF,n=9) or granulocyte-macrophage colony-stimulating factor (GM-CSF,n=8) was given subcutaneously for 5 days (5 μg/kg/day). Controls (n=5) received no growth factor. Laboratory parameters and side effects were monitored for 8 days. Within 24 h, both cytokines led to a rapid increase of leukocytes, the majority of which were granulocytes. Compared with the controls (n=5), the increase on day 5 in the G-CSF/GM-CSF groups was 37-/10-fold (CD34+ cells), 5.2-/2.4-fold (leukocytes), 7.2-/3.0-fold (granulocytes), 7.4-/4.4-fold (monocytes), 1.7-/1.1-fold (lymphocytes), 9.8-/2.7-fold (basophils), 2.3-/9.6-fold (eosinophils), and 1.9-/1.6-fold (reticulocytes). The mobilization of myeloblasts, promyelocytes, myelocytes, and metamyelocytes coincided with the pronounced increase of CD34+ PBPC observed on day 4. Serum levels of uric acid (UA) and lactic dehydrogenase (LDH) increased under G-CSF, and platelets decreased after G-CSF discontinuation. Rash at the injection site occurred in 50% of the GM-CSF-treated volunteers. Seven volunteers in the GM-CSF group and six in the G-CSF cohort complained of flu-like symptoms, including musculoskeletal pain. We conclude that, in terms of tolerance and mobilization of CD34+ cells and leukocytes, G-CSF is superior to GM-CSF, but higher levels of UA and LDH and late decrease in platelets make monitoring of these parameters necessary.

Key words G-CSFGM-CSFMobilizationHealthy adultsClinical and laboratory parameters

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • G. Fischmeister
    • 1
  • M. Kurz
    • 1
  • O. A. Haas
    • 1
  • M. Micksche
    • 1
  • P. Buchinger
    • 1
  • D. Printz
    • 1
  • G. Ressmann
    • 1
  • T. Stroebel
    • 1
  • C. Peters
    • 1
  • G. Fritsch
    • 1
  • H. Gadner
    • 1
  1. 1.Children's Cancer Research Institute (CCRI), St. Anna Kinderspital, Kinderspitalgasse 6, A-1090 Vienna, Austria e-mail: Gadner@ccri.univie.ac.at, Tel: 0043-01-40170, Fax: 0043-01-40170-70AT
  2. 2.Dept. of Transfusion Medicine, University of Vienna, AustriaAT
  3. 3.Institute of Tumor Biology/Cancer Research, University of Vienna, AustriaAT