ORIGINAL ARTICLE

Annals of Hematology

, Volume 74, Issue 5, pp 231-238

Preclinical evaluation of biotin labeling for red cell survival testing

  • G. Hoffmann-FezerAffiliated withGSF – National Research Center for Environment and Health, Institute of Immunology, Marchionionistrasse 25, D-81377 Munich, Germany
  • , C. TrastlAffiliated withGSF – National Research Center for Environment and Health, Institute of Immunology, Marchionionistrasse 25, D-81377 Munich, Germany
  • , W. BeiskerAffiliated withDepartment of Flow Cytometry, GSF, Munich, Germany
  • , D. BergAffiliated withInstitute of Radiobiology, GSF, Munich, Germany
  • , J. ObermaierAffiliated withInstitute of Medical Informatics and Health Services Research, GSF, Munich, Germany
  • , W. KesslerAffiliated withInstitute of Toxicology, GSF, Munich, Germany
  • , J. MysliwietzAffiliated withGSF – National Research Center for Environment and Health, Institute of Immunology, Marchionionistrasse 25, D-81377 Munich, Germany
  • , M. SchummAffiliated withInstitute of Clinical Hematology, GSF, Munich, Germany
  • , J. FilserAffiliated withInstitute of Toxicology, GSF, Munich, Germany
    • , S. ThierfelderAffiliated withGSF – National Research Center for Environment and Health, Institute of Immunology, Marchionionistrasse 25, D-81377 Munich, Germany

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Abstract

 Biotin labeling of red cells was tested in dogs as a preclinical study for cell survival. Red cells were labeled with either spacered Biotin-X-NHS (BxNHS) or water-soluble biotin compounds. After reinfusion, biotinylated red cells were detected in small blood samples (5 μl) with flow cytometry. Improved BxNHS labeling allows an easy detection of positive red cells for almost 100 days, whereas labeling with watersoluble compounds – despite strong labeling during the first days – results in a decrease of label, which prevented a discrimation between labeled and negative cells after about 4 weeks. When biotin labeling of red cells was compared with 51Cr labeling, slopes of red cell survival were quite similar after the latter were corrected for elution. Survival slopes were linear, and the mean survival time was t=93d. In two blood-donor dogs the slopes of red cell survival where log linear and the mean survival time was t=45d. In conclusion, BxNHS, but not the water-soluble biotin compounds, is a good nonradioactive, nontoxic alternative for red cell survival studies. No health hazards are to be expected from the very low dose of Dimethylformamide, which is used as a solvent for biotin-x-NHS.

Key words Red cell survival Biotinylated red cells Biotin labeled erythrocytes Dog red cells