Annals of Hematology

, Volume 91, Issue 12, pp 1845–1853

Analysis of SNP rs16754 of WT1 gene in a series of de novo acute myeloid leukemia patients

Authors

    • Department of HematologyHospital Universitari i Politècnic La Fe
    • Department of HematologyHospital Universitari La Fe
  • Esperanza Such
    • Department of HematologyHospital Universitari i Politècnic La Fe
  • Jose Cervera
    • Department of HematologyHospital Universitari i Politècnic La Fe
  • Eva Barragán
    • Laboratory of Molecular Biology, Department of Medical PathologyHospital Universitario La Fe
  • Antonio Jiménez-Velasco
    • Department of HematologyHospital Universitario Carlos Haya
  • Sandra Dolz
    • Laboratory of Molecular Biology, Department of Medical PathologyHospital Universitario La Fe
  • Mariam Ibáñez
    • Department of HematologyHospital Universitari i Politècnic La Fe
  • Inés Gómez-Seguí
    • Department of HematologyHospital Universitari i Politècnic La Fe
  • María López-Pavía
    • Department of HematologyHospital Universitari i Politècnic La Fe
  • Marta Llop
    • Laboratory of Molecular Biology, Department of Medical PathologyHospital Universitario La Fe
  • Óscar Fuster
    • Laboratory of Molecular Biology, Department of Medical PathologyHospital Universitario La Fe
  • Silvestre Oltra
    • Genetic UnitHospital Universitario La Fe
  • Federico Moscardó
    • Department of HematologyHospital Universitari i Politècnic La Fe
  • David Martínez-Cuadrón
    • Department of HematologyHospital Universitari i Politècnic La Fe
  • M. Leonor Senent
    • Department of HematologyHospital Universitari i Politècnic La Fe
  • Adriana Gascón
    • Department of HematologyHospital Universitari i Politècnic La Fe
  • Pau Montesinos
    • Department of HematologyHospital Universitari i Politècnic La Fe
  • Guillermo Martín
    • Department of HematologyHospital Universitari i Politècnic La Fe
  • Pascual Bolufer
    • Laboratory of Molecular Biology, Department of Medical PathologyHospital Universitario La Fe
    • Department of HematologyHospital Universitari i Politècnic La Fe
    • Facultad de MedicinaUniversidad de Valencia
Original Article

DOI: 10.1007/s00277-012-1596-x

Cite this article as:
Luna, I., Such, E., Cervera, J. et al. Ann Hematol (2012) 91: 1845. doi:10.1007/s00277-012-1596-x

Abstract

The single nucleotide polymorphism (SNP) rs16754 of the WT1 gene has been previously described as a possible prognostic marker in normal karyotype acute myeloid leukemia (AML) patients. Nevertheless, the findings in this field are not always reproducible in different series. One hundred and seventy-five adult de novo AML patients were screened with two different methods for the detection of SNP rs16754: high-resolution melting (HRM) and FRET hybridization probes. Direct sequencing was used to validate both techniques. The SNP was detected in 52 out of 175 patients (30 %), both by HRM and hybridization probes. Direct sequencing confirmed that every positive sample in the screening methods had a variation in the DNA sequence. Patients with the wild-type genotype (WT1AA) for the SNP rs16754 were significantly younger than those with the heterozygous WT1AG genotype. No other difference was observed for baseline characteristic or outcome between patients with or without the SNP. Both techniques are equally reliable and reproducible as screening methods for the detection of the SNP rs16754, allowing for the selection of those samples that will need to be sequenced. We were unable to confirm the suggested favorable outcome of SNP rs16754 in de novo AML.

Keywords

Acute myeloid leukemiaSNP rs16754 of WT1 gene

Supplementary material

277_2012_1596_MOESM1_ESM.doc (883 kb)
ESM 1(DOC 883 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2012