Annals of Hematology

, Volume 89, Issue 12, pp 1197–1205

Effects of neuropeptide substance P on the expression of adhesion molecules in cord blood hematopoietic stem cells

  • Somayeh Shahrokhi
  • Kamran Alimoghaddam
  • Massoumeh Ebtekar
  • Ali Akbar Pourfathollah
  • Maryam Kheirandish
  • Alireza Ardjmand
  • Ardeshir Ghavamzadeh
Original Article

DOI: 10.1007/s00277-010-1006-1

Cite this article as:
Shahrokhi, S., Alimoghaddam, K., Ebtekar, M. et al. Ann Hematol (2010) 89: 1197. doi:10.1007/s00277-010-1006-1

Abstract

Modulation of adhesion molecules expression on the surface of cord blood (CB) CD34+ cells may assist in overcoming the delay in cord blood engraftment. Likewise, utilization of diverse growth factors such as neuropeptides could also be helpful. Therefore, we aimed to assess the role of Substance P (SP) along with a cytokine cocktail on CB CD34+ adhesion molecule expression. CB CD34+ cells were cultured in a serum-free media containing different concentrations of SP in combination with a cytokine cocktail (SCF, FL, TPO, IL-3, and IL-6). Expression of adhesion molecules CXCR4, CD44, CD49e, and CD62L was analyzed after 7 and/or 11 days of cell cultivation. Additionally, the colonogenic capacity of cells was analyzed by colony formation unit assay. Our results show an enhanced percentage of CD34+cells with CXCR4, CD44, and CD62L on day 7, as compared with control. Furthermore, an increase in frequency was observed for CD49e+ CD34+cells by day 7 in both test and control groups compared with day 0. Colonogenic assays show occurrence of more total colony formation and immature progenitor cells in SP-treated cells. Our study indicates that SP could act as an effective modulator for expression of cell adhesion molecules.

Keywords

Adhesion moleculeCord bloodStem cellSubstance p

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Somayeh Shahrokhi
    • 1
  • Kamran Alimoghaddam
    • 2
    • 3
  • Massoumeh Ebtekar
    • 6
  • Ali Akbar Pourfathollah
    • 1
  • Maryam Kheirandish
    • 4
  • Alireza Ardjmand
    • 5
  • Ardeshir Ghavamzadeh
    • 2
  1. 1.Department of Immunology, School of Medical SciencesTarbiat Modares UniversityTehranIran
  2. 2.Hematology, Oncology and Stem Cell Transplantation Research CenterTehran University of Medical SciencesTehranIran
  3. 3.Cord Blood Bank of Iran, Hematology, Oncology and Stem Cell Transplantation Research CenterTehran University of Medical SciencesTehranIran
  4. 4.Research Center, Department of ImmunologyIranian Blood Transfusion OrganizationTehranIran
  5. 5.Cancer Research Unit, School of Biomedical Science, Faculty of HealthThe University of NewcastleNewcastleAustralia
  6. 6.Department of Immunology, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran