Annals of Hematology

, Volume 89, Issue 7, pp 715–723

Poor potential of proliferation and differentiation in bone marrow mesenchymal stem cells derived from children with severe aplastic anemia

Authors

  • Yu-Hua Chao
    • Department of PediatricsTungs’ Taichung MetroHarbor Hospital
    • Graduate Institute of Clinical Medical ScienceChina Medical University
  • Ching-Tien Peng
    • Department of PediatricsChina Medical University Hospital
    • Department of Biotechnology and BioinformaticsAsia University
  • Horng-Jyh Harn
    • Center for NeuropsychiatryChina Medical University Hospital
    • Department of PathologyChina Medical University Hospital
  • Chin-Kan Chan
    • Graduate Institute of Clinical Medical ScienceChang Gung University
    • Department of PediatricsTaoyuan General Hospital
    • Department of PediatricsChina Medical University Hospital
    • Stem Cell Research Laboratory, Department of Medical ResearchChina Medical University Hospital
Original Article

DOI: 10.1007/s00277-009-0892-6

Cite this article as:
Chao, Y., Peng, C., Harn, H. et al. Ann Hematol (2010) 89: 715. doi:10.1007/s00277-009-0892-6

Abstract

The pathogenesis of severe aplastic anemia (SAA) has not been completely understood, and insufficiency of the hematopoietic microenvironment can be an important factor. Here, we compared the basic properties of mesenchymal stem cells (MSCs), a major component of bone marrow microenvironment, from five SAA children with those of MSCs from five controls. Although MSCs from SAA children and controls were similar in morphology and immunophenotypic profile, SAA MSCs had slower expansion rate and smaller cumulative population doubling (1.83 ± 1.21 vs 3.36 ± 0.87; p = 0.046), indicating lower proliferative capacity. After osteogenic induction, SAA MSCs showed lower alkaline phosphatase activity (optical density, 1.46 ± 0.04 vs 2.27 ± 0.32; p = 0.013), less intense von Kossa staining, and lower gene expression of core binding factor α1 (0.0015 ± 0.0005 vs 0.0056 ± 0.0017; p = 0.013). Following adipogenic induction, SAA MSCs showed less intense Oil red O staining (optical density, 0.86 ± 0.22 vs 1.73 ± 0.42; p = 0.013) and lower lipoprotein lipase expression (0.0105 ± 0.0074 vs 0.0527 ± 0.0254; p = 0.013). These findings provided evidence that defects in bone marrow MSCs of SAA children do exist.

Keywords

Aplastic anemiaMesenchymal stem cellsHematopoiesisBone marrow failureMicroenvironment

Copyright information

© Springer-Verlag 2010