Annals of Hematology

, Volume 89, Issue 7, pp 663–669

Expression of pSTAT5 predicts FLT3 internal tandem duplications in acute myeloid leukemia

  • Ellen Christina Obermann
  • Caroline Arber
  • Martine Jotterand
  • Andre Tichelli
  • Petra Hirschmann
  • Alexandar Tzankov
Original Article

DOI: 10.1007/s00277-009-0890-8

Cite this article as:
Obermann, E.C., Arber, C., Jotterand, M. et al. Ann Hematol (2010) 89: 663. doi:10.1007/s00277-009-0890-8
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Abstract

Mutations of the Fms-like tyrosine kinase 3 (FLT3) can be detected in a significant number of acute myeloid leukemias (AML). Seventy-five cases of acute myeloid leukemia were evaluated for FLT3-internal tandem duplications (ITD) by polymerase chain reaction. Paraffin-embedded formalin-fixed trephine biopsies of these cases were evaluated for expression of phosphorylated signal transducer and activator of transcription 1 (pSTAT1), pSTAT3, and pSTAT5. Specific expression of pSTAT5 was proven in leukemic blasts in situ by double staining with a blast-specific marker. Expression of pSTAT5 in ≥1% of blasts was highly predictive of FLT3-ITD. Neither expression of pSTAT1 nor pSTAT3 were associated with FLT3 mutations. Altogether we conclude that pSTAT5 expression can precisely be assessed by immunohistochemistry in routinely processed bone marrow trephines, STAT5 is highly likely the preferred second messenger of FLT3-mediated signaling in AML, and expression of pSTAT5 is predictive of FLT3-ITD.

Keywords

Acute myeloid leukemia FLT3 FLT3-ITD pSTAT5 

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Ellen Christina Obermann
    • 1
  • Caroline Arber
    • 2
  • Martine Jotterand
    • 3
  • Andre Tichelli
    • 2
  • Petra Hirschmann
    • 1
  • Alexandar Tzankov
    • 1
  1. 1.Institute of PathologyUniversity of BaselBaselSwitzerland
  2. 2.Hematology UnitUniversity of BaselBaselSwitzerland
  3. 3.Unité cytogénétique du cancerCentre Hospitalier Universitaire VaudoisLausanneSwitzerland

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