Annals of Hematology

, 89:155

Aberrant expression of Notch signaling molecules in patients with immune thrombocytopenic purpura

  • Daoxin Ma
  • Jianjian Dai
  • Xiaojuan Zhu
  • Shuxin Yan
  • Ping Zhao
  • Jingru Zhang
  • Yuanyuan Zhu
  • Jianzhi Sun
  • Jun Peng
  • Chunyan Ji
  • Ming Hou
Original Article

DOI: 10.1007/s00277-009-0790-y

Cite this article as:
Ma, D., Dai, J., Zhu, X. et al. Ann Hematol (2010) 89: 155. doi:10.1007/s00277-009-0790-y

Abstract

To investigate the role of Notch signaling pathway in immune thrombocytopenic purpura (ITP), we measured the expression of 11 Notch pathway molecules in ITP patients and evaluated their clinical relevance. Real-time reverse transcriptase polymerase chain reaction results showed there was aberrant expression of some Notch molecules in ITP. Notch1 and Notch3 expression elevated, while Notch2 decreased statistically in ITP patients. As for Notch ligands, only DLL1 was found downregulated in ITP. The expression of Notch target gene, Hes1, was also upregulated. In accordance with the mRNA level, Notch1 and Hes1 protein expression was also found elevated by Western blot. Immunocytochemistry showed that Notch1 expressed highly in the cytomembrane, cytoplasm, and part of cellular nucleus for ITP while weak in cytomembrane for controls, and Hes1 of ITP was found expressed higher in cellular nucleus than that of controls. Our findings suggest that the aberrant expression profile of Notch pathway may be involved in ITP, and blockage of Notch1 pathway is likely a promising therapeutic concept.

Keywords

ExpressionDetectionImmune thrombocytopenic purpuraNotch

Supplementary material

277_2009_790_MOESM1_ESM.xls (32 kb)
Supplementary Material: Results (22 ITP and 22 controls) (XLS 31 kb)

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Daoxin Ma
    • 1
    • 2
  • Jianjian Dai
    • 1
    • 2
  • Xiaojuan Zhu
    • 1
    • 2
  • Shuxin Yan
    • 1
    • 2
  • Ping Zhao
    • 1
    • 3
  • Jingru Zhang
    • 1
    • 2
  • Yuanyuan Zhu
    • 1
    • 2
  • Jianzhi Sun
    • 1
    • 2
  • Jun Peng
    • 1
    • 2
  • Chunyan Ji
    • 1
    • 2
  • Ming Hou
    • 1
    • 2
  1. 1.Department of HematologyQilu Hospital, Shandong UniversityJinanPeople’s Republic of China
  2. 2.The Key Laboratory of Cardiovascular Remodeling and Function ResearchChinese Ministry of Education and Chinese Ministry of HealthJinanPeople’s Republic of China
  3. 3.Department of Pediatrics, Jinan Central Hospital affiliated to Shandong UniversityJinanPeople’s Republic of China