Annals of Hematology

, Volume 88, Issue 7, pp 623–628

No telomere shortening in marrow stroma from patients with MDS

  • A. Mario Marcondes
  • Steven Bair
  • Peter S. Rabinovitch
  • Ted Gooley
  • H. Joachim Deeg
  • Rosana Risques
Original Article

DOI: 10.1007/s00277-008-0649-7

Cite this article as:
Marcondes, A.M., Bair, S., Rabinovitch, P.S. et al. Ann Hematol (2009) 88: 623. doi:10.1007/s00277-008-0649-7
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Abstract

Telomere shortening with age may lead to genomic instability and an increased risk of cancer. Given the role of the microenvironment in the pathophysiology of the myelodysplastic syndrome (MDS), primarily a disease of older age, we determined telomere length in primary cultured marrow stroma cells using quantitative fluorescent in situ hybridization (qFISH) and quantitative polymerase chain reaction (qPCR). qFISH showed comparable rates of decrease in telomere length with age in MDS patients and age-matched healthy controls. Telomere length assessment by qPCR showed similar results. These findings suggest a lack of significant differences between MDS patients and healthy controls in terms of telomere stability in marrow stroma in contrast to that observed in hematopoietic cells. In conclusion, this demonstrates that, although MDS stroma cells and hematopoietic cells share the same microenvironment, the stromal cells do not share the processes that contribute to accelerated telomere attrition, suggesting that stromal cell proliferative potential is not limiting in MDS.

Keywords

Marrow stroma Telomere Stroma qFISH 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • A. Mario Marcondes
    • 1
    • 2
    • 4
  • Steven Bair
    • 1
  • Peter S. Rabinovitch
    • 2
  • Ted Gooley
    • 1
  • H. Joachim Deeg
    • 1
    • 3
  • Rosana Risques
    • 2
  1. 1.Clinical Research DivisionFred Hutchinson Cancer Research CenterSeattleUSA
  2. 2.Department of PathologyUniversity of WashingtonSeattleUSA
  3. 3.Department of Medicine, Division of OncologyUniversity of WashingtonSeattleUSA
  4. 4.Fred Hutchinson Cancer Research CenterSeattleUSA

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