Annals of Hematology

, Volume 87, Issue 6, pp 451–457

Tissue factor procoagulant activity of plasma microparticles in patients with cancer-associated disseminated intravascular coagulation

  • Florian Langer
  • Brigitte Spath
  • Katja Haubold
  • Katharina Holstein
  • Guy Marx
  • Jan Wierecky
  • Tim H. Brümmendorf
  • Judith Dierlamm
  • Carsten Bokemeyer
  • Barbara Eifrig
Original Article

DOI: 10.1007/s00277-008-0446-3

Cite this article as:
Langer, F., Spath, B., Haubold, K. et al. Ann Hematol (2008) 87: 451. doi:10.1007/s00277-008-0446-3

Abstract

Tissue factor (TF) expressed on sub-cellular membrane vesicles, so-called plasma microparticles (MPs), has recently emerged as a potential key player in intravascular coagulation activation in various disease states. In this report, we demonstrate significantly increased levels of TF-specific procoagulant activity (PCA) of plasma MPs in five patients presenting with overt disseminated intravascular coagulation (DIC) due to an underlying malignancy, including non-small-cell lung cancer (n = 1), melanoma (n = 1), prostate cancer (n = 2), and acute promyelocytic leukemia (n = 1). Clotting experiments on available tumor cell samples suggested that cancer cells were a potential source of circulating TF-positive MPs at least in three of the five patients. Furthermore, follow-up plasma samples from two surviving patients revealed that response of their malignancies to specific anti-cancer therapy was paralleled by resolution of overt DIC and a significant decline in MP-associated TF PCA. Levels of plasma TF antigen, as assessed by an enzyme-linked immunosorbent assay, were also increased at presentation albeit to a lesser extent compared to MP-associated TF PCA, likely due to insufficient solubilization of the phospholipid-incorporated full-length TF molecule by the detergent. In summary, our findings suggest that MP-associated TF PCA may play an important pathogenic role in the evolution of overt DIC in various types of malignancy.

Keywords

Tissue factor Microparticles Cancer Disseminated intravascular coagulation 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Florian Langer
    • 1
  • Brigitte Spath
    • 1
  • Katja Haubold
    • 1
  • Katharina Holstein
    • 1
  • Guy Marx
    • 2
  • Jan Wierecky
    • 1
  • Tim H. Brümmendorf
    • 1
  • Judith Dierlamm
    • 1
  • Carsten Bokemeyer
    • 1
  • Barbara Eifrig
    • 1
  1. 1.Onkologisches Zentrum, II. Medizinische Klinik und PoliklinikUniversitätsklinikum Hamburg-EppendorfHamburgGermany
  2. 2.Institut für Klinische ChemieUniversitätsklinikum Hamburg-EppendorfHamburgGermany

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