Annals of Hematology

, Volume 87, Issue 4, pp 257–262

Detection of CD55- and CD59-deficient granulocytic populations in patients with myelodysplastic syndrome

Authors

  • Georgia Kaiafa
    • First Propedeutic Department of Internal Medicine, AHEPA HospitalAristotle University
  • Athanasios Papadopoulos
    • First Propedeutic Department of Internal Medicine, AHEPA HospitalAristotle University
    • First Propedeutic Department of Internal Medicine, AHEPA HospitalAristotle University
    • S. Kiriakidi 1, AHEPA Hospital
  • Zoi Saouli
    • First Propedeutic Department of Internal Medicine, AHEPA HospitalAristotle University
  • Christos Savopoulos
    • First Propedeutic Department of Internal Medicine, AHEPA HospitalAristotle University
  • Niki Tsesmeli
    • First Propedeutic Department of Internal Medicine, AHEPA HospitalAristotle University
  • Zisis Kontoninas
    • First Propedeutic Department of Internal Medicine, AHEPA HospitalAristotle University
  • Anastasia Chatzinikolaou
    • First Propedeutic Department of Internal Medicine, AHEPA HospitalAristotle University
  • Vasiliki Tsavdaridou
    • First Propedeutic Department of Internal Medicine, AHEPA HospitalAristotle University
  • Ioannis Klonizakis
    • First Propedeutic Department of Internal Medicine, AHEPA HospitalAristotle University
  • Apostolos Hatzitolios
    • First Propedeutic Department of Internal Medicine, AHEPA HospitalAristotle University
Original Article

DOI: 10.1007/s00277-007-0420-5

Cite this article as:
Kaiafa, G., Papadopoulos, A., Ntaios, G. et al. Ann Hematol (2008) 87: 257. doi:10.1007/s00277-007-0420-5

Abstract

Paroxysmal nocturnal hemoglobinuria (PNH) is characterized by absence of CD55 and CD59 from the surface of affected cells. PNH has been associated with myelodysplastic syndromes (MDS). The aim of our study was to estimate the prevalence of the PNH clone in MDS patients by detecting CD55 and CD59 deficiency. We studied 90 MDS patients: 19 patients with RA, 15 with refractory anemia with ringed sideroblasts (RARS), 18 with refractory anemia with excess of blasts (RAEB), 17 with refractory anemia with excess of blasts in transformation (RAEB-t), and 21 with chronic myelomonocytic leukemia (CMML). Twenty healthy individuals were also studied as the control group. We studied the PNH clone on granulocytes of these patients with the aid of flow cytometry. CD55- and CD59-deficient granulocytic populations were detected in 15.5% of MDS patients compared to 2.8% of normal individuals. Among the subgroups of the study, significant difference was present in three cases: (1) between CMML and control, (2) between CMML and RA, and (3) between CMML and RARS. These data indicate a possible association between PNH phenotype and MDS. MDS patients of worse prognosis (CMML) express more strongly the PNH clone compared to those of better prognosis (RA and RARS). Perhaps, the examination of MDS patients for the PNH clone by flow cytometry could provide us with a valuable prognostic tool.

Keywords

Paroxysmal nocturnal hemoglobinuriaCD55 (DAF) and CD59 (MIRL) moleculesGPI anchor proteinsFlow cytometryMyelodysplastic syndromes

Copyright information

© Springer-Verlag 2007