Annals of Hematology

, Volume 84, Issue 9, pp 572–577

Expression of interferon-γ and tumor necrosis factor-α in bone marrow T cells and their levels in bone marrow plasma in patients with aplastic anemia

Original Article

DOI: 10.1007/s00277-005-1022-8

Cite this article as:
Dubey, S., Shukla, P. & Nityanand, S. Ann Hematol (2005) 84: 572. doi:10.1007/s00277-005-1022-8

Abstract

Immune-mediated stem cell damage has been postulated to be responsible for disease initiation and progression in aplastic anemia (AA). It is hypothesized that T lymphocytes play a major role in destroying the bone marrow (BM) stem cells of AA patients by infiltrating the BM and secreting excessive levels of anti-hematopoietic cytokines, interferon-gamma (IFN-γ), and tumor necrosis factor-alpha (TNF-α). We undertook this study to assess the pathogenic significance of anti-hematopoietic cytokines such as IFN-γ and TNF-α in BM T cells and plasma of AA patients. Significantly elevated levels of IFN-γ and TNF-α were found in the BM plasma of AA patients compared to controls (p=0.05 and 0.006, respectively). Intracellular IFN-γ and not TNF-α in BM CD3+ T cells of AA patients was significantly higher compared to controls (p=0.04 and p=0.2, respectively). A follow-up analysis of expression of these cytokines in BM T cells and their levels in BM plasma in five AA patients before and 180 days (6 months) after antithymocyte globulin (ATG) and cyclosporin A (CsA) therapy showed a decline 180 days after therapy compared to pre-therapy. We thus conclude that increased production of both IFN-γ and TNF-α in the BM may contribute to disease pathogenesis in AA and ATG therapy may induce hematological remission by suppressing the elevated levels of IFN-γ and TNF-α in AA BM.

Keywords

Aplastic anemia (AA) Interferon-gamma (IFN-γ) Tumor necrosis factor-alpha (TNF-α) Hematopoiesis Apoptosis 

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Shweta Dubey
    • 1
  • Priyanka Shukla
    • 2
  • Soniya Nityanand
    • 2
  1. 1.Department of ImmunologySanjay Gandhi Post-Graduate Institute of Medical Sciences (SGPGIMS)LucknowIndia
  2. 2.Department of HematologySanjay Gandhi Post-Graduate Institute of Medical Sciences (SGPGIMS)LucknowIndia

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