Annals of Hematology

, Volume 81, Issue 3, pp 170–173

Use of recombinant human deoxyribonuclease (DNase) for processing of a thawed umbilical cord blood transplant in a patient with relapsed acute lymphoblastic leukemia

  • H. Eichler
  • C. Beck
  • F. Bernard
  • P. Bugert
  • H. Klüter
Case report

DOI: 10.1007/s00277-001-0419-2

Cite this article as:
Eichler, H., Beck, C., Bernard, F. et al. Ann Hematol (2002) 81: 170. doi:10.1007/s00277-001-0419-2

Abstract.

This case report describes for the first time the use of a recombinant human enzyme deoxyribonuclease (rhDNase) containing solution for the processing of a thawed umbilical cord blood (UCB) unit prior to successful transplantation to avoid cell losses by clotting phenomena. A 6-year-old boy received an unrelated 2/6 HLA antigen mismatched UCB transplant for high-risk Burkitt type acute lymphoblastic leukemia. The UCB unit was provided as a volume-reduced sample after buffy coat separation with a final volume of 36 ml. To avoid the loss of nucleated cells due to cell clumping during thawing procedure cells were washed with a solution containing the rhDNase. No visible clotting of the resuspended unit occurred, and the patient was transplanted with 2.9×107 nucleated cells/kg body weight without any acute or chronic side effects due to rhDNase. On day +35, PCR analysis of bone marrow aspirate showed complete chimerism, and the child engrafted with an absolute neutrophil count greater than 0.5×109/l on day +47. Platelet transfusion independence was achieved on day +120. In conclusion, the supplementation of rhDNase to the washing and resuspension solutions of a thawed UCB unit is effective to prevent cell losses prior to transplantation. However, further investigations must be performed to confirm the safety of this procedure.

Umbilical cord blood rhDNase Stem cell transplantation Cord blood processing

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • H. Eichler
    • 1
  • C. Beck
    • 2
  • F. Bernard
    • 3
  • P. Bugert
    • 2
  • H. Klüter
    • 2
  1. 1.Institut für Transfusionsmedizin und Immunologie Mannheim, DRK-Blutspendedienst Baden-Württemberg-Hessen, Friedrich-Ebert-Strasse 107, 68167 Mannheim, GermanyGermany
  2. 2.Institute of Transfusion Medicine and Immunology, Red Cross Blood Service of Baden-Württemberg-Hessen, College of Clinical Medicine Mannheim, University of Heidelberg, Mannheim, GermanyGermany
  3. 3.Hématologie Pédiatrie, Hôpital Arnaud de Villeneuve, Montpellier, FranceFrance