Building a Comprehensive Genomic Program for Hepatocellular Carcinoma
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
Hepatocellular carcinoma (HCC) is the most common primary liver cancer, causing approximately 660,000 deaths worldwide annually. The preferred treatment of HCC is surgical resection or orthotopic liver transplantation (OLT) for patients meeting specific criteria. For patients outside these criteria, options are limited and include medical therapy, radiofrequency ablation, chemoembolization, or palliative measures, and these result in poor outcomes. Various centers at Baylor are elucidating the genomics of HCC to improve treatment options, with a focus on three etiologies: hepatitis C virus, hepatitis B virus, and non-viral.
Through collaborative efforts, we have established an effective specimen biobanking protocol, and we are using several techniques to analyze HCC, including whole genome sequencing, whole exome sequencing, gene-specific analysis, gene expression, and epigenetic analysis.
We have completed whole genome sequencing on two patient samples, whole exome sequencing on 47 patient samples, gene-specific analysis on 94 patient samples, gene expression on 4 patient samples, and epigenetic analysis on 1 patient sample.
We hope to use these results to define novel genetic therapeutic strategies that may work in conjunction with surgical approaches to improve long-term patient and graft survival rates in patients with HCC. We also aim to provide a functional framework of a comprehensive program for genomic analysis that may be imitated by other institutions and for other tumors in the global quest toward personalized genomic medicine.
- Geller DA, Goss JA, Tsung A (2009) Liver, Chap 31. In: Brunicardi FC, Andersen DK, Billiar TR, Dunn DL, Hunter JG, Matthews JB, Pollock RE (eds) Schwartz’s principles of surgery, 9th edn. McGraw-Hill, New York. http://www.accesssurgery.com/content.aspx?aID=5018142. Accessed 31 Dec 2010
- Jarnagin WR (2009) Liver & portal venous system, Chap 24. In: Doherty GM (ed) Current diagnosis & treatment: surgery, 13th edn. McGraw-Hill, New York. http://www.accesssurgery.com/content.aspx?aID=5216497. Accessed 31 Dec 2010
- El-Serag HB, Rudolph KL (2007) Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology 132:2557–2576 CrossRef
- Tsoulfas G, Kawai T, Elias N et al (2010) Long-term experience with liver transplantation for hepatocellular carcinoma. J Gastroenterol [Epub ahead of print]
- Island ER, Pomposelli J, Pomfret EA et al (2005) Twenty-year experience with liver transplantation for hepatocellular carcinoma. Arch Surg 140:353–358 CrossRef
- Mazzaferro V, Regalia E, Doci R et al (1996) Liver transplantation for the treatment of small hepatocellular carcinomas in patients with cirrhosis. N Engl J Med 334:693–699 CrossRef
- Fan H-L, Chen T-W, Hsieh C-B et al (2010) Liver transplantation is an alternative treatment of hepatocellular carcinoma beyond the Milan criteria. Am J Surg 200:252–257 CrossRef
- Zimmerman MA, Ghobrial RM, Tong MJ et al (2008) Recurrence of hepatocellular carcinoma following liver transplantation: a review of preoperative and postoperative prognostic indicators. Arch Surg 143:182–188 CrossRef
- Rodriguez JA, Guiteau JJ, Nazareth L et al (2009) Sequencing the full-length of the phosphatase and tensin homolog (PTEN) gene in hepatocellular carcinoma (HCC) using the 454 GS20 and Illumina GA DNA sequencing platforms. World J Surg 33:647–652 CrossRef
- Voidonikolas G, Kreml SS, Chen C et al (2009) Basic principles and technologies for deciphering the genetic map of cancer. World J Surg 33:615–629 CrossRef
- Sanger F, Coulson AR (1975) A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J Mol Biol 94:441–448 CrossRef
- The International Human Genome Sequencing Consortium (2004) Finishing the euchromatic sequence of the human genome. Nature 431:931–945 CrossRef
- Levy S, Sutton G, Ng PC et al (2007) The diploid genome sequence of an individual human. PLoS Biol 5:2113–2144 CrossRef
- Wheeler DA, Srinivasan M, Egholm M et al (2008) The complete genome of an individual by massively parallel DNA sequencing. Nature 452:872–877 CrossRef
- Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467 CrossRef
- Building a Comprehensive Genomic Program for Hepatocellular Carcinoma
World Journal of Surgery
Volume 35, Issue 8 , pp 1746-1750
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Industry Sectors
- Author Affiliations
- 1. Michael E. DeBakey Department of Surgery, Baylor College of Medicine, One Baylor Plaza, Jewish Bldg #404D, Houston, TX, 77030, USA
- 2. Department of Molecular and Human Genetics, Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Alkek Bldg #N1419/1519/1619, Houston, TX, 77030, USA
- 3. The Liver Center, Division of Abdominal Transplantation, Michael E. DeBakey Department of Surgery, Baylor College of Medicine, 1709 Dryden Road, Suite #1500, Houston, TX, 77030, USA
- 4. Michael E. DeBakey Department of Surgery, Baylor College of Medicine, 1709 Dryden Road, Suite #1500, Houston, TX, 77030, USA