Molecular Testing for Somatic Mutations Improves the Accuracy of Thyroid Fine-needle Aspiration Biopsy
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- Moses, W., Weng, J., Sansano, I. et al. World J Surg (2010) 34: 2589. doi:10.1007/s00268-010-0720-0
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Thyroid fine-needle aspiration (FNA) biopsy is indeterminate or suspicious in up to 30% of cases and these patients are commonly subjected to at least a diagnostic hemithyroidectomy. If malignant on histology, a completion thyroidectomy is usually performed, which may be associated with higher morbidity. To determine the clinical utility of genetic testing in thyroid FNA biopsy, we conducted a prospective clinical trial.
Four hundred seventeen patients with 455 thyroid nodules were enrolled and had genetic testing for common somatic mutations (BRAF, NRAS, KRAS) and gene rearrangements (RET/PTC1, RET/PTC3, RAS, TRK1) by PCR and direct sequencing and by nested PCR, respectively. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of genetic testing in thyroid FNA biopsy were determined based on the histologic diagnosis.
One hundred twenty-five of 455 thyroid nodule FNA biopsies were indeterminate or suspicious on cytologic examination. Overall, 50 mutations were identified (23 BRAF, 4 RET/PTC1, 2 RET/PTC3, 21 NRAS) in the thyroid FNA biopsies. There were significantly more mutations detected in malignant thyroid nodules than in benign (P = 0.0001). For thyroid FNA biopsies that were indeterminate or suspicious, genetic testing had a sensitivity of 12%, specificity of 98%, PPV of 38%, and NPV of 65%.
Genetic testing for somatic mutations in thyroid FNA biopsy samples is feasible and identifies a subset of malignant thyroid neoplasms that are indeterminate or suspicious on FNA biopsy. Genetic testing for common somatic genetic alterations thus could allow for more definitive initial thyroidectomy in those with positive results.
The number of patients requiring clinical evaluation of thyroid nodules has dramatically increased over the past three decades due to the increasing number of thyroid incidentalomas . Fine-needle aspiration (FNA) biopsy is the most accurate and cost-effective preoperative test to distinguish benign from malignant thyroid nodules. Most thyroid nodules are benign and only about 5% of them have malignant features on FNA biopsy and cytologic examination. Unfortunately, FNA biopsy is inconclusive in about 30% of all thyroid nodule biopsies because the cytologic features are indeterminate (follicular and Hürthle neoplasm), suspicious for malignancy but not completely diagnostic, or nondiagnostic (insufficient cells) upon cytologic examination. These patients are typically subjected to a diagnostic thyroidectomy to exclude a thyroid cancer diagnosis. Approximately 20% of patients with FNA biopsy showing indeterminate cytologic features will have thyroid cancer on histologic examination and may require a completion thyroidectomy.
Although FNA biopsy is accurate for benign and malignant interpretations, up to 10% of benign cytologic results may be false negatives and result in delay in diagnosis and treatment . Also, significant discordance in the thyroid FNA biopsy cytologic interpretation has been reported on secondary review, especially for indeterminate, nondiagnostic, and suspicious findings . The discordance in thyroid FNA biopsy interpretation can have significant management ramifications, ranging from patients avoiding thyroidectomy to allowing for more definitive initial surgical treatment in some cases. In order to decrease the need for unnecessary procedures and the additional costs incurred for diagnostic and completion thyroidectomies, new approaches to improving the diagnostic accuracy of FNA biopsy are needed.
Thyroid cancer marker research has been an active area with numerous approaches and candidate markers emerging but very few markers have been evaluated in a clinical trial to find whether they would be useful in clinical practice . Molecular testing for common somatic mutations is one promising approach that has emerged because about two-thirds of thyroid cancers of follicular cell origin have one of the common genetic alterations (BRAF and RAS point mutations and RET/PTC and NTRK1 rearrangements), the genetic changes that occur are usually mutually exclusive events, and most of these genetic changes are specific to thyroid cancer of follicular cell origin (BRAF mutation and RET/PTC rearrangements). Somatic genetic alterations in thyroid cancer of follicular cell origin occur in genes involved in the tyrosine kinase signaling pathway (tyrosine kinase receptors: RET/PTC and NTRK1; intracellular signaling proteins: HRAS, KRAS, NRAS, and BRAF). The most prevalent genetic alteration is a point mutation in BRAF (V600E), which is present in approximately 40% of classic papillary thyroid cancers. RET/PTC and NTRK1 chromosomal rearrangements are present in about 30% and up to 15% of classic papillary thyroid cancers, respectively . Hotspot mutations in NRAS and KRAS are more common in follicular thyroid carcinoma but also occur in benign thyroid neoplasm at a lower rate.
Several studies have reported that testing for the common somatic genetic changes in thyroid cancer may be useful for detecting thyroid cancer in indeterminate and suspicious thyroid FNA biopsy results [5–15]. Most of these studies were retrospective, tested for a limited number of genetic changes, and/or had a relatively small group of indeterminate, suspicious, and nondiagnostic FNA cytology groups. In fact, at the National Cancer Institute State of the Science Conference on thyroid FNA biopsy, it was suggested that although promising, there was limited data to recommend molecular testing for common somatic genetic alterations as an ancillary test to thyroid FNA biopsy for indeterminate and suspicious cytologic findings .
In this prospective clinical trial, we wanted to determine the feasibility and accuracy of molecular testing for a comprehensive panel of somatic genetic alterations associated with thyroid cancer of follicular cell origin in clinical thyroid FNA biopsy samples. We evaluated 455 thyroid nodule FNA biopsy samples from 417 patients using PCR and direct sequencing for hotspot mutations in BRAF, NRAS, and KRAS, and nested PCR for RET/PTC1, RET/PTC3, and NTRK1 rearrangements.
Materials and methods
RNA extraction and cDNA synthesis
Total RNA was extracted using the TRIzol reagent according to the manufacturer’s protocol. The amount of total RNA was determined by spectrophotometry using NanoDrop 3.0 (NanoDrop, Inc., Wilmington, DE). One microgram of total RNA was reverse transcribed using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA).
PCR detection of somatic genetic alterations
Detection of hotspot mutations in BRAF, KRAS, and NRAS
PCR primers for BRAF, KRAS, and NRAS hotspot mutation analyses
Primer sequences (5′–3′)
b 5′-AGCGGATAACAATTTCACACAGGC CAA AAA TTTAATCAGTGGA-3′
a 5′-TGTAAAACGACGGCCAGTCATAATGCTTGCTCTGA TAG GA-3′
b 5′-AGCGGATAACAATTTCACACAGGCCAA AAATTTAATCAGTGGA-3′
PCR products were prepared for direct sequencing using enzymatic reactions of Exonuclease I (USB Corp., Cleveland, OH) and Shrimp Alkaline Phosphatase (USB Corp) to digest excess primers and dephosphorylate any free nucleotides. This was carried out using the iCycler at 37°C for 30 min followed by 15 min at 80°C. Samples were then prepared with reverse primers for each hotspot mutation and sequenced using the ABI BigDye v3.1 dye terminator sequencing chemistry with the ABI PRISM 3730xl capillary DNA analyzer. The sequences were analyzed to determine mutation status using Mutation Surveyor v3.10 (SoftGenetics, State College, PA).
Detection of RET/PTC1, RET/PTC3, and NTRK1 chromosomal rearrangements
PCR primers for RET/PTC1, RET/PTC3, and NTRK1 chromosomal rearrangements
Primer sequences (5′–3′)
Nested primer sequences
a 5′-GCT GGA GAC CTA CAA ACT GA-3′
a 5′-ACA AAC TGA AGT GCA AGG CA-3′
b 5′-GTT GCC TTG ACC ACT TTT C-3′
b 5′-GCC TTG ACC ACT ACT TTT CCA AA-3′
a 5′-AAG CAA ACC TGC CAG TGG-3′
a 5′-CCT GCC AGT GGT TAT CAA GC-3′
b 5′-CTT TCA GCA TCT TCA CGG-3′
b 5′-GGC CAC CGT GGT GTA CCC TG-3′
The sensitivity, specificity, PPV, and NPV of genetic testing in thyroid FNA biopsy were determined based on the histologic diagnosis. Cases with no mutation and benign on histology were considered “true negative.” Cases with a mutation and malignant on histology were considered “true positive.” The overall accuracy was determined using all of the genetic alterations in combination and in a subset analysis excluding those mutations that may be present in benign thyroid neoplasm (NRAS and KRAS). The genetic testing was performed blinded to the FNA biopsy and histologic diagnosis.
Demographic and clinical characteristics of study cohort
Mean ± SD or no.
51 ± 15
Family history of thyroid disease
History of head and neck irradiation
FNA biopsy diagnosisa
Molecular testing for all the common genetic alterations in combination had a sensitivity of 38%, specificity of 65%, PPV of 42%, and NPV of 65%. When cases positive for NRAS mutations were excluded because the mutations can be present in benign thyroid neoplasm, the sensitivity was 12% and specificity was 98% (2 false-positive results). One BRAF V600E mutation was detected in a benign dominant thyroid nodule that had microscopic papillary thyroid cancer. One RET/PTC3 rearrangement was detected in a benign thyroid nodule in the background of chronic lymphocytic thyroiditis.
In cases with indeterminate and suspicious thyroid FNA biopsy cytologic diagnosis, 17 cases (12%) were positive for BRAF V600E mutation and RET/PTC1 or RET/PTC3 rearrangements (Fig. 2). For initial treatment, 5 had a total thyroidectomy and 12 underwent only a diagnostic thyroidectomy. In these 12 cases, the presence of the somatic mutations specific for papillary thyroid cancer could have been used to allow a more definitive initial treatment as all of these patients went on to have a completion thyroidectomy based on the malignant histologic diagnosis.
FNA biopsy has remained the most accurate and cost-effective preoperative diagnostic test to distinguish benign from malignant thyroid nodules. However, given that up to 30% of thyroid nodule FNA biopsy results are inconclusive, many patients still undergo a diagnostic thyroidectomy to exclude a cancer diagnosis, and they sometimes need a completion thyroidectomy. Moreover, the number of patients found to have a thyroid incidentaloma is increasing, and the number of patients with inconclusive FNA biopsy results is even larger. For these reasons, new ways to improve the diagnostic accuracy of thyroid FNA biopsy have been actively investigated. We used molecular testing for common somatic mutations in thyroid cancer of follicular cell origin to determine the accuracy and feasibility of prospectively testing clinical thyroid FNA biopsy samples. Based on the histologic diagnosis, we found a significantly higher rate of mutations in malignant than in benign thyroid neoplasms. However, molecular testing for a combination of mutations had low sensitivity and specificity. Exclusion of NRAS mutations increased specificity but lowered sensitivity. In 137 cases with indeterminate or suspicious thyroid FNA cytologic diagnosis, 17 cases were positive for BRAF V600E, RET/PTC1, or RET/PTC3 mutations, and in 12 cases a more definitive initial thyroidectomy procedure could have been performed based on the molecular testing result.
Mutation analysis for single or a combination of somatic mutations associated with thyroid cancer of follicular cell origin has been reported to improve the diagnostic accuracy ranging from 16 to 48% of cases with indeterminate and/or suspicious thyroid FNA cytologic diagnosis [5–11]. We found that in 12% of indeterminate and suspicious thyroid FNA results, mutation analysis could have allowed for a more definitive initial surgical treatment. The wide range in diagnostic accuracy improvement associated with molecular testing of thyroid FNA biopsies could be due to several reasons, including differences in FNA classification and diagnosis among centers, different rates of malignancy based on cytologic categories, thyroid nodule sampling, preservation of FNA biopsies, and the number of genetic alterations for which the biopsy was tested. We may also have observed a lower rate in sensitivity of combined mutation analysis because the remnant material from the thyroid FNA was used for molecular testing. Also, RNA was used to detect RET/PTC and NTRK1 rearrangements which may be expressed only at low levels, while they are more readily detectable if genomic DNA was used to detect rearrangements of these genes. Testing for PAX8/PPARγ rearrangement and HRAS mutation may also improve the sensitivity of molecular testing, but they are also present in benign follicular tumors at a relatively high rate and thus were not included in the panel of genetic alterations tested for in our study.
The strengths of our study are the large study cohort, prospective and blinded mutation analysis without clinical knowledge of the cytologic diagnosis, and the use of the remaining clinical FNA biopsy material. The follow-up time was not sufficient to determine accurately the false-negative rate of thyroid FNA biopsy and see if those benign cases with NRAS mutation would ever progress to a malignant tumor. Also, the use of RNA instead of DNA may have reduced the detection rate of gene rearrangements detected but the study was instituted for a dual purpose of evaluating a multigene expression assay .
In summary, molecular testing for a panel of common somatic genetic alterations in thyroid FNA biopsy with indeterminate or suspicious cytologic features is feasible and could allow for more definitive initial surgical therapy in a subset of cases. However, the NPV of genetic testing is too low to reduce the need for a diagnostic thyroidectomy in patients with indeterminate or suspicious thyroid FNA biopsies.
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