World Journal of Surgery

, Volume 30, Issue 12, pp 2217–2226

Effect of Glucocorticoid Pretreatment on Oxidative Liver Injury and Survival in Jaundiced Rats with Endotoxin Cholangitis

  • Chi Wei Lee
  • Jiin Haur Chuang
  • Pei Wen Wang
  • Nyuk Kong Chang
  • Hsiu Chuan Wang
  • Chao Cheng Huang
  • Mao Meng Tiao
  • Sing Kai Lo
Article

DOI: 10.1007/s00268-006-0143-0

Cite this article as:
Lee, C.W., Chuang, J.H., Wang, P.W. et al. World J. Surg. (2006) 30: 2217. doi:10.1007/s00268-006-0143-0

Abstract

Introduction

Biliary tract infection is associated with high mortality. This study investigated the effect of glucocorticoid pretreatment on lipopolysaccharide (LPS)-induced cholangitis.

Methods

Rats undergoing either sham operation or ligation of the extrahepatic bile duct (BDL) for 2 weeks were randomly assigned to receive intravenous injections of dexamethasone (DX) or normal saline (NS) prior to infusing LPS into the biliary tract. The plasma levels of tumor necrosis factor-α (TNFα), chemokines monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-2 (MIP-2) as well as liver mRNA expression of MCP-1 and MIP-2 were determined. Infiltration of monocytes, Kupffer cells, and neutrophils in rat liver were studied with immunohistochemistry. Oxidative liver injury was measured by the malondialdehyde (MDA) content.

Results

Dexamethasone pretreatment resulted in significantly decreased plasma levels of TNFα at 1 hour, MCP-1 and MIP-2 at 2 and 3 hours, and decreased liver MCP-1 mRNA expression at 3 hours following LPS infusion in BDL-DX rats than in BDL-NS rats. The number of inflammatory cells in the liver was significantly different between sham- and BDL-treated rats but was not affected by DX pretreatment. Pretreatment with DX resulted in significantly decreased liver MDA contents in the BDL-DX group than that in the BDL-NS group. Jaundiced rats pretreated with 5 mg DX prior to infusion of 1 g of LPS were 6.8 times more likely to survive than those that were not pretreated.

Conclusions

Pretreatment of jaundiced, LPS-treated rats with a supraphysiological dose of dexamethasone may rescue their lives by suppression of chemokine expression and alleviation of oxidative liver injury.

Copyright information

© Société Internationale de Chirurgie 2006

Authors and Affiliations

  • Chi Wei Lee
    • 1
    • 2
  • Jiin Haur Chuang
    • 2
    • 3
  • Pei Wen Wang
    • 4
  • Nyuk Kong Chang
    • 3
  • Hsiu Chuan Wang
    • 3
  • Chao Cheng Huang
    • 6
  • Mao Meng Tiao
    • 1
  • Sing Kai Lo
    • 7
  1. 1.Department of Emergency MedicineE-Da Hospital, I-Shou UniversityYan-chau ShiangTaiwan
  2. 2.Graduate Institute of Clinical Medical SciencesChang Gung UniversityNiao-Sung HsiangTaiwan
  3. 3.Department of SurgeryChang Gung Memorial HospitalNiao-Sung HsiangTaiwan
  4. 4.Department of Internal MedicineChang Gung Memorial HospitalNiao-Sung HsiangTaiwan
  5. 5.Department of PathologyChang Gung Memorial HospitalNiao-Sung HsiangTaiwan
  6. 6.Department of PediatricsChang Gung Memorial HospitalNiao-Sung HsiangTaiwan
  7. 7.Faculty of Health and Behavioral SciencesDeakin UniversityBurwood VictoriaAustralia