Aesthetic Plastic Surgery

, 35:756

Acellular Dermal Matrix Seeded with Adipose-Derived Stem Cells as a Subcutaneous Implant

  • Hakan Orbay
  • Yoshihiro Takami
  • Hiko Hyakusoku
  • Hiroshi Mizuno
Original Article

DOI: 10.1007/s00266-011-9683-2

Cite this article as:
Orbay, H., Takami, Y., Hyakusoku, H. et al. Aesth Plast Surg (2011) 35: 756. doi:10.1007/s00266-011-9683-2

Abstract

Background

Soft tissue augmentation using autologous materials usually is associated with high resorption rates. To obtain more durable soft tissue filler, acellular dermal matrix (ADM) was seeded with adipose-derived stem cells (ASCs) in this study.

Methods

For ADM preparation, split-thickness skin was obtained from the dorsum of two Fischer rats. Harvested skin was acellularized to obtain ADM and subsequently seeded in vitro with ASCs isolated from the same rats. Subcutaneous soft tissue augmentation was carried out in the dorsal area of 20 Fischer rats. The implant materials were ADM (group 1), ADM with ASCs (group 2), collagen type 1 gel (group 3), and collagen type 1 gel with ASCs (group 4). Each specimen was harvested after 8 weeks for quantitative evaluation of thickness, vascular density, and collagen content.

Results

Histologic analysis showed that ASCs were successfully seeded onto ADM. The thickness of the implanted material and the vascular density were highest 8 weeks postoperatively in group 2. The subpanniculus layer became significantly thicker in group 3 than in group 4. The collagen content was significantly higher in group 2 than in the other groups.

Conclusion

These findings suggest that ADM seeded with ASCs forms the best implant material in terms of volume maintenance, vascular density, and collagen content.

Keywords

Acellular dermal matrixAdipose-derived stem cellsAugmentationSoft tissue

Copyright information

© Springer Science+Business Media, LLC and International Society of Aesthetic Plastic Surgery 2011

Authors and Affiliations

  • Hakan Orbay
    • 1
  • Yoshihiro Takami
    • 1
  • Hiko Hyakusoku
    • 1
  • Hiroshi Mizuno
    • 2
  1. 1.Department of Plastic and Reconstructive SurgeryNippon Medical SchoolTokyoJapan
  2. 2.Department of Plastic and Reconstructive SurgeryJuntendo University School of MedicineTokyoJapan