111Indium-oxine labelling for evaluating the homing process of autologous osteoblasts implanted percutaneously in atrophic nonunion fractures
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The aim of the study was to control the in vivo localisation of implanted cells in cell-based therapies. Labelling cells with 111indium-oxine is one of the most interesting methods proposed. We evaluated this method in the setting of autologous osteoblast implantation in nonunion fractures.
An in vitro study of osteoblasts was conducted after 111indium-oxine labelling. Radioactivity retention and viability, proliferation and the ability to produce alkaline phosphatase were evaluated in a seven-day culture. In vivo labelling of implanted osteoblastic cells was conducted during a therapeutic trial of atrophic nonunion fractures, with the leakage outside the nonunion site and local uptake evolution at four, 24 and 48 hour being studied.
The mean labelling efficiency for osteoprogenitors was 78.8 ± 4.6 %. The intracellular retention was 89.4 ± 2.1 % at three hours and 67.3 ± 4.7 % at 18 hours. The viability assessed at three hours was 93.7 ± 0.6 %. After seven days of culture, morphology and alkaline phosphatase staining were similar for both labelled and unlabelled control cells, although the proliferation rate was decreased in the labelled cells. Some local intraosseous leakage was observed in four of 17 cases. All patients showed uptake at the injection site, with four having no other uptake. Four patients showed additional uptake in the bladder, liver and spleen, while 11 patients had additional uptake in the lungs in addition to the bladder, liver and spleen. The activity ratios (injection site/body) were 48 ± 28 % at four hours, 40 ± 25 % at 24 hours and 35 ± 25 % at 48 hours. After correcting for decay, the activity within the injection site was 82 ± 15 % at 24 hours and 69 ± 11 % at 48 hours compared with the activity measured at four hours. No relationship was found between uptake and radiological bone repair.
The 111indium-oxine labelling appears to be a good method for monitoring the behaviour of the osteoblastic cells after their implantation in atrophic nonunion fractures.
- Gangji V, Hauzeur JP, Matos C, De Maertelaer V, Toungouz M, Lambermont M (2004) Treatment of osteonecrosis of the femoral head with implantation of autologous bone-marrow cells. A pilot study. J Bone Joint Surg Am 86-A:1153–1160
- Hauzeur JP, Gangji V (2010) Phases 1–3 clinical trials using adult stem cells in osteonecrosis and nonunion fractures. Stem Cells Int 26:410170. doi:10.4061/2010/410170
- Gangji V, De Maertelaer V, Hauzeur JP (2011) Autologous bone marrow cell implantation in the treatment of non-traumatic osteonecrosis of the femoral head: five year follow-up of a prospective controlled study. Bone 49(5):1005–1009. doi:10.1016/j.bone.2011.07.032 CrossRef
- Megas P (2005) Classification of non-union. Injury 36(Suppl 4):S30–S37. doi:10.1016/j.injury.2005.10.008
- Hauzeur JP, Orloff S, Taverne-Verbanck J, Pasteels JL (1986) Diagnostic of aseptic osteonecrosis of the femoral head by percutaneous transtrochanterian needle biopsy. Clin Rheumatol 5:346–358 CrossRef
- McAfee JG, Thakur ML (1976) Survey of radioactive agents for in vitro labeling of phagocytic leukocytes. II. Particles. J Nucl Med 17(6):488–492
- Botti C, Negri DR, Seregni E, Ramakrishna V, Arienti F, Maffioli L, Lombardo C, Bogni A, Pascali C, Crippa F, Massaron S, Remonti F, Nerini-Molteni S, Canevari S, Bombardieri E (1997) Comparison of three different methods for radiolabelling human activated T lymphocytes. Eur J Nucl Med 24(5):497–504
- Jonsson AC, Jönsson B, Strand SE, Grafström G, Spanne P (1996) Cell survival after Auger electron emission from stable intracellular indium exposed to monochromatic synchrotron radiation. Acta Oncol 35:947–952 CrossRef
- Bindslev L, Haack-Sørensen M, Bisgaard K, Kragh L, Mortensen S, Hesse B, Kjaer A, Kastrup J (2006) Labelling of human mesenchymal stem cells with indium-111 for SPECT imaging: effect on cell proliferation and differentiation. Eur J Nucl Med Mol Imaging 33(10):1171–1177. doi:10.1007/s00259-006-0093-7 CrossRef
- Nowak B, Weber C, Schober A, Zeiffer U, Liehn EA, von Hundelshausen P, Reinartz P, Schaefer WM, Buell U (2007) Indium-111 oxine labelling affects the cellular integrity of haematopoietic progenitor cells. Eur J Nucl Med Mol Imaging 34(5):715–721. doi:10.1007/s00259-006-0275-3 CrossRef
- Gholamrezanezhad A, Mirpour S, Ardekani JM, Bagheri M, Alimoghadam K, Yarmand S, Malekzadeh R (2009) Cytotoxicity of 111In-oxine on mesenchymal stem cells: a time-dependent adverse effect. Nucl Med Commun 30(3):210–216. doi:10.1097/MNM.0b013e328318b328 CrossRef
- Cui J, Wahl RL, Shen T, Fisher SJ, Recker E, Ginsburg D, Long MW (1999) Bone marrow cell trafficking following intravenous administration. Br J Haematol 107(4):895–902. doi:10.1046/j.1365-2141.1999.01779.x CrossRef
- 111Indium-oxine labelling for evaluating the homing process of autologous osteoblasts implanted percutaneously in atrophic nonunion fractures
Volume 37, Issue 1 , pp 131-136
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- 1. Department of Rheumatology, CHU Sart Tilman, University of Liège, Liège, Belgium
- 2. Department of Nuclear Medicine, CHU Sart Tilman, University of Liège, Liège, Belgium
- 3. Department of Nuclear Medicine, Erasme University Hospital, University of Brussels, Brussels, Belgium
- 4. Department of Orthopaedic Surgery, CHU Sart Tilman, University of Liège, Liège, Belgium
- 5. Laboratory of Cell and Gene Therapy, CHU Sart Tilman, Liège, Belgium