International Orthopaedics

, Volume 34, Issue 7, pp 1059–1068

Use of small interfering ribonucleic acids to inhibit the adipogenic effect of alcohol on human bone marrow-derived mesenchymal cells

Authors

  • Qiang Huang
    • Department of Orthopedics, West China HospitalSichuan University
    • Department of Orthopedics, West China HospitalSichuan University
    • Department of Orthopedics, West China HospitalSichuan University
  • Zhi-yu Chen
    • Department of AnesthesiologyXinhua Hospital affiliated Shanghai University Medical College
  • Guang-lin Wang
    • Department of Orthopedics, West China HospitalSichuan University
  • Bin Shen
    • Department of Orthopedics, West China HospitalSichuan University
  • Jing Yang
    • Department of Orthopedics, West China HospitalSichuan University
  • Zong-ke Zhou
    • Department of Orthopedics, West China HospitalSichuan University
  • Qing-quan Kong
    • Department of Orthopedics, West China HospitalSichuan University
Original Paper

DOI: 10.1007/s00264-009-0914-y

Cite this article as:
Huang, Q., Zhang, H., Pei, F. et al. International Orthopaedics (SICOT) (2010) 34: 1059. doi:10.1007/s00264-009-0914-y
  • 99 Views

Abstract

This study tested the potential of small interfering RNAs (siRNA) targeting human peroxisome proliferator activated receptor gamma (PPARγ) to repress the adipogenic effect of alcohol on human bone marrow-derived mesenchymal cells (hBMSCs). hBMSCs were cultured from hip replacement surgery patients (n = 10). PPARγ-siRNA was transiently transfected into hBMSCs cultured in ostogenic media containing 50 mM alcohol by using a liposome-based strategy. Oil red O staining was used to test the development of differentiated adipocytes, and Alizarin red staining was used to test mineral deposition. Marker genes of adipogenesis (PPARγ2 and aP2) and osteogenesis (Osf2/Cbfa1) were examined through real time RT-PCR and Western blot, respectively. Collagen type I, alkaline phosphatase and osteocalcin protein synthesis of cultures were also assayed. Data were presented as mean ± SD. Differences between the means of the treatment groups were determined with ANOVA. PPARγ-siRNA transfection resulted in significantly lower adipocyte number, increased matrix mineralisation, repressed adipogenic gene markers, up-regulated osteogenic gene marker and bone matrix protein synthesis in the PPARγ-siRNA group compared to controls (P < 0.05). PPARγ-siRNA is a useful strategy to inhibit the adipogenic effect and the osteogenic repression of alcohol on hBMSCs. This may be a novel therapeutic intervention for osteopenic disorders in alcoholism and other conditions.

Copyright information

© Springer-Verlag 2009