Cancer Immunology, Immunotherapy

, Volume 50, Issue 11, pp 604–614

Differential expression of granulysin and perforin by NK cells in cancer patients and correlation of impaired granulysin expression with progression of cancer

  • Atsuko Kishi
  • Yasushi Takamori
  • Kazuyuki Ogawa
  • Shoichi Takano
  • Shuji Tomita
  • Mari Tanigawa
  • Makiko Niman
  • Tsunataro Kishida
  • Setsuya Fujita
Original Article

DOI: 10.1007/s002620100228

Cite this article as:
Kishi, A., Takamori, Y., Ogawa, K. et al. Cancer Immunol Immunother (2002) 50: 604. doi:10.1007/s002620100228

Abstract.

Granulysin has been identified as an effector molecule co-localized with perforin in the cytotoxic granules of cytotoxic T lymphocytes and natural killer (NK) cells, and has been reported to kill intracellular pathogens in infected cells in the presence of perforin and to induce a cytotoxic effect against tumor cells. The aim of the present study was to elucidate whether intracellular expression of granulysin and perforin by NK cells might be associated with progression of cancer. Flow cytometric analysis demonstrated high levels of perforin and granulysin expression by CD3 CD16+ cells in healthy controls. In contrast, cancer patients exhibited significantly decreased levels of granulysin expression (P<0.005), despite having equally high levels of perforin expression in comparison with healthy controls. The tumor-free patients expressed granulysin at levels similar to healthy controls, while the progressive tumor-bearing patients expressed remarkably lower levels of granulysin compared to healthy controls (P<0.0001). Similarly, patients with an advanced performance status had significantly fewer granulysin-positive NK cells than healthy controls. Meanwhile, a considerable number of the tumor-bearing patients showed a decrease in the number of circulating NK cells, and a correlation between impaired granulysin expression and reduced circulating NK cells was observed. These findings suggest that the tumor-bearing patients with impaired granulysin expression were in an immunosuppressive state. In conclusion, impaired expression of granulysin by NK cells correlates with progression of cancer, and determination of granulysin expression might prove informative for assessing the immunological condition of cancer patients.

Granulysin Perforin NK cells Cancer

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Atsuko Kishi
    • 1
  • Yasushi Takamori
    • 3
  • Kazuyuki Ogawa
    • 3
  • Shoichi Takano
    • 3
  • Shuji Tomita
    • 1
  • Mari Tanigawa
    • 1
  • Makiko Niman
    • 1
  • Tsunataro Kishida
    • 1
  • Setsuya Fujita
    • 1
  1. 1.Louis Pasteur Center for Medical Research, 103-5, Tanaka Monzen-cho, Sakyo-ku, Kyoto, 606-8225, Japan
  2. 2.Department of Microbiology, Kyoto Prefectural University of Medicine, Kyoto, 602–0841, Japan
  3. 3.B.M.L. R&D Center, 1361-1, Matoba, Kawagoe, Saitama, 350-11, Japan