Cancer Immunology, Immunotherapy

, Volume 48, Issue 5, pp 239–246

Establishment of gp100 and MART-1/Melan-A-specific cytotoxic T lymphocyte clones using in vitro immunization against preselected highly immunogenic melanoma cell clones

  • Alexei F. Kirkin
  • Per thor Straten
  • Mia Riise Hansen
  • Annette Barfoed
  • Karine N. Dzhandzhugazyan
  • Jesper Zeuthen
ORIGINAL ARTICLE

DOI: 10.1007/s002620050571

Cite this article as:
Kirkin, A., thor Straten, P., Hansen, M. et al. Cancer Immunol Immunother (1999) 48: 239. doi:10.1007/s002620050571

Abstract

The induction of an in vitro T cell response against tumour-associated antigens with subsequent expansion of the individual cytotoxic T lymphocyte (CTL) clones still is not routine and the only tumour-associated antigen that has been found to easily induce the establishment of CTL clones is the MART-1/Melan-A antigen. In this paper, we describe a new approach for in vitro immunization based on the use of preselected melanoma cell clones. The human melanoma cell subline FM3.P was cloned and the immunological properties of individual clones were compared. Melanoma cell clone FM3.29, having a high level of expression of melanoma differentiation antigens, as well as high levels of the HLA class I and class II antigens and adhesion molecules, was used for the establishment of a CTL line that was subsequently cloned. For optimization of the conditions of growth of established CTL clones, a particular melanoma subline FM3.D/40 was selected for supporting the proliferation of CTL clones. The majority of the established CTL clones recognized the melanoma-associated differentiation antigens gp100 and MART-1/Melan-A. Epitope analysis indicated that two different epitopes derived from gp100 (154–162 and 280–288) and a single epitope from MART-1/Melan-A (27–35) were recognized by these CTL clones. The gp100-specific CTL clones were found to be significantly more sensitive to the culture conditions than the MART-1/Melan-A-specific CTL clones. In addition, the presence of excess peptide in the culture medium induced autokilling of the gp100-specific, but not the MART-1/Melan-A-specific CTL clones. Taken together, these results demonstrate that, by careful preselection of melanoma cell lines and clones both for the induction of CTL line from patients' peripheral blood lymphocytes and subsequent cloning, it is possible to obtain a large number of stable CTL clones even against such an inherently “difficult” differentiation antigen as gp100.

Key words Melanoma-associated antigensCTL clonesMelanoma cell clones

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Alexei F. Kirkin
    • 1
  • Per thor Straten
    • 1
  • Mia Riise Hansen
    • 1
  • Annette Barfoed
    • 1
  • Karine N. Dzhandzhugazyan
    • 1
  • Jesper Zeuthen
    • 1
  1. 1.Department of Tumour Cell Biology, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, DK-2100 Copenhagen, Denmark e-mail: jz@bio.cancer.dk Tel.: +45-35-25-75-00 Fax: +45-35-25-77-21DK