Cancer Immunology, Immunotherapy

, Volume 42, Issue 1, pp 47–54

Phenotypic, functional and molecular analysis of lymphocytes associated with bladder cancer

  • Silvia Bruno
  • Anna Maria Machí
  • Claudia Semino
  • Maurizio Meta
  • Marco Ponte
  • Marco Varaldo
  • Antonio Curotto
  • Guido Ferlazzo
  • Andrea Decensi
  • G. Melioli
ORIGINAL ARTICLE

DOI: 10.1007/s002620050250

Cite this article as:
Bruno, S., Machí, A., Semino, C. et al. Cancer Immunol Immunother (1996) 42: 47. doi:10.1007/s002620050250

Abstract

 Bladder-washing-derived lymphocytes (BWDL) from 67 patients with bladder cancer were studied. The large majority of samples contained a pure population of T lymphocytes, whereas B and NK cells were absent. A comparative analysis of bladder lymphocytes and peripheral blood lymphocytes (PBL), collected in parallel, showed that BWDL significantly differed from PBL. In vitro cultures of bladder lymphocytes were attempted on 21 samples but in vitro expansion was only possible on six patients treated with bacillus Calmette-Guérin (BCG). This finding indicates that BWDL are characterized by a severe proliferative defect. Nevertheless, the addition of BCG on bladder lymphocytes expanded in vitro enhanced their proliferation, suggesting that this population is sensitized against BCG antigen(s). The analysis of T cell receptor restriction patterns showed that bladder lymphocytes from patients under BCG treatment were oligoclonal. A possible explanation for the efficiency of the immune response and good clinical outcome in patients treated with BCG could be found in the high homology between some BCG antigens and human heat-shock proteins, which are overexpressed in transformed cells.

Key words LymphocytesBacillus Calmette-GuérinBladder cancer

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Silvia Bruno
    • 1
  • Anna Maria Machí
    • 2
  • Claudia Semino
    • 1
  • Maurizio Meta
    • 1
  • Marco Ponte
    • 1
  • Marco Varaldo
    • 3
  • Antonio Curotto
    • 4
  • Guido Ferlazzo
    • 5
  • Andrea Decensi
    • 6
  • G. Melioli
    • 1
  1. 1.Servizio di Patologia Clinica, Istituto Nazionale per la Ricerca sul Cancro, Largo Rosanna Benzi, 10, I-16136 Genova, ItalyIT
  2. 2.Laboratorio di Analisi, Ospedale S. Martino, Genova, ItalyIT
  3. 3.Divisione di Urologia, Ospedale Gallino, Genova, ItalyIT
  4. 4.Clinica Urologica, Universitá di Genova, Genova, ItalyIT
  5. 5.Istituto di Oncologia, Universitá di Messina, Messina, ItalyIT
  6. 6.Servizio di Oncologia Medica II, Istituto Nazionale per la Ricerca sul Cancro, Genova, ItalyIT