Abstract
The standard treatment of high-grade glioma presents a combination of radiotherapy, chemotherapy and surgery. Immunotherapy is proposed as a potential adjunct to standard cytotoxic regimens to target remaining microscopic disease following resection. We have shown ex vivo expanded/activated γδ T cells to be a promising innate lymphocyte therapy based on their recognition of stress antigens expressed on gliomas. However, successful integration of γδ T cell therapy protocols requires understanding the efficacy and safety of adoptively transferred immune cells in the post-treatment environment. The unique features of γδ T cell product and the environment (hypoxia, inflammation) can affect levels of expression of key cell receptors and secreted factors and either promote or hinder the feasibility of γδ T cell therapy. We investigated the potential for the γδ T cells to injure normal brain tissue that may have been stressed by treatment. We evaluated γδ T cell toxicity by assessing actual and correlative toxicity indicators in several available models including: (1) expression of stress markers on normal primary human astrocytes (as surrogate for brain parenchyma) after irradiation and temozolomide treatment, (2) cytotoxicity of γδ T cells on normal and irradiated primary astrocytes, (3) microglial activation and expression of stress-induced ligands in mouse brain after whole-brain irradiation and (4) expression of stress-induced markers on human brain tumors and on normal brain tissue. The lack of expression of stress-induced ligands in all tested models suggests that γδ T cell therapy is safe for brain tumor patients who undergo standard cytotoxic therapies.
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Abbreviations
- BrdU:
-
Bromodeoxyuridine
- CAR:
-
Chimeric antigen receptor
- CTL:
-
Cytotoxic T lymphocytes
- DAPI:
-
4′,6-diamidino-2-phenylindole, nuclear counterstaining dye
- DDR:
-
DNA damage response
- E:T:
-
Effector-to-target ratio
- FFPE:
-
Formalin-fixed paraffin-embedded specimens
- GBM:
-
Glioblastoma multiforme
- γδ T:
-
Gamma delta T cells
- IHC:
-
Immunohistochemistry
- IL:
-
Interleukin
- LAK:
-
Lymphokine-activated killer cells
- NK:
-
Natural killer cells
- TCR:
-
T cell receptor
- TMZ:
-
Temozolomide
- TNFα:
-
Tumor necrosis factor α
- UAB:
-
University of Alabama at Birmingham
- ZOL:
-
Zoledronic acid
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Acknowledgments
We thank Dr. Mitchell S. Berger, Department of Neurological Surgery, University of California, San Francisco for making available FFPE samples of human brain tumors. Support from Elsa U Pardee Foundation (Lawrence S. Lamb) is acknowledged and appreciated.
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The authors declare that they have no conflict of interest.
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Pereboeva, L., Harkins, L., Wong, S. et al. The safety of allogeneic innate lymphocyte therapy for glioma patients with prior cranial irradiation. Cancer Immunol Immunother 64, 551–562 (2015). https://doi.org/10.1007/s00262-015-1662-z
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DOI: https://doi.org/10.1007/s00262-015-1662-z