Cancer Immunology, Immunotherapy

, Volume 62, Issue 10, pp 1619–1628

DEAD/H (Asp–Glu–Ala–Asp/His) box polypeptide 3, X-linked is an immunogenic target of cancer stem cells

  • Jun Koshio
  • Hiroshi Kagamu
  • Koichiro Nozaki
  • Yu Saida
  • Tomohiro Tanaka
  • Satoshi Shoji
  • Natsue Igarashi
  • Satoru Miura
  • Masaaki Okajima
  • Satoshi Watanabe
  • Hirohisa Yoshizawa
  • Ichiei Narita
Original Article

DOI: 10.1007/s00262-013-1467-x

Cite this article as:
Koshio, J., Kagamu, H., Nozaki, K. et al. Cancer Immunol Immunother (2013) 62: 1619. doi:10.1007/s00262-013-1467-x

Abstract

Accumulating evidence suggests that most solid malignancies consist of heterogeneous tumor cells and that a relatively small subpopulation, which shares biological features with stem cells, survives through potentially lethal stresses such as chemotherapy and radiation treatment. Since the survival of this subpopulation of cancer stem cells (CSC) plays a critical role in recurrence, it must be eradicated in order to cure cancer. We previously reported that vaccination with CD133+ murine melanoma cells exhibiting biological CSC features induced CSC-specific effector T cells. These were capable of eradicating CD133+ tumor cells in vivo, thereby curing the parental tumor. In the current study, we indicated that DEAD/H (Asp–Glu–Ala–Asp/His) box polypeptide 3, X-linked (DDX3X) is an immunogenic protein preferentially expressed in CD133+ tumor cells. Vaccination with DDX3X primed specific T cells, resulting in protective and therapeutic antitumor immunity. The DDX3X-primed CD4+ T cells produced CD133+ tumor-specific IFNγ and IL-17 and mediated potent antitumor therapeutic efficacy. DDX3X is expressed in various human cancer cells, including lung, colon, and breast cancer cells. These results suggest that anti-DDX3X immunotherapy is a promising treatment option in efforts to eradicate CSC in the clinical setting.

Keywords

Tumor immunology Helper T cells Cancer stem cells DDX3X 

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Jun Koshio
    • 1
  • Hiroshi Kagamu
    • 1
  • Koichiro Nozaki
    • 1
  • Yu Saida
    • 1
  • Tomohiro Tanaka
    • 1
  • Satoshi Shoji
    • 1
  • Natsue Igarashi
    • 1
  • Satoru Miura
    • 1
  • Masaaki Okajima
    • 1
  • Satoshi Watanabe
    • 2
  • Hirohisa Yoshizawa
    • 2
  • Ichiei Narita
    • 1
  1. 1.Division of Respiratory Medicine, Department of Homeostatic Regulation and Development, Course for Biological Functions and Medical Control, Graduate School of Medical and Dental SciencesNiigata UniversityNiigataJapan
  2. 2.Bioscience Medical Research CenterNiigata University Medical and Dental HospitalNiigataJapan

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