Cancer Immunology, Immunotherapy

, Volume 62, Issue 6, pp 1123–1130

Elevated level of peripheral CD8+CD28 T lymphocytes are an independent predictor of progression-free survival in patients with metastatic breast cancer during the course of chemotherapy

Authors

  • Guohong Song
    • Department of Medical Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Peking University Cancer Hospital and Institute
  • Xiaoli Wang
    • Department of Medical Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Peking University Cancer Hospital and Institute
    • Capital Medical University Cancer CenterBeijing Shijitan Hospital
  • Jun Jia
    • Department of Medical Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Peking University Cancer Hospital and Institute
  • Yanhua Yuan
    • Department of Medical Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Peking University Cancer Hospital and Institute
  • Fengling Wan
    • Department of Medical Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Peking University Cancer Hospital and Institute
  • Xinna Zhou
    • Department of Medical Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Peking University Cancer Hospital and Institute
    • Capital Medical University Cancer CenterBeijing Shijitan Hospital
  • Huabing Yang
    • Department of Medical Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Peking University Cancer Hospital and Institute
    • Capital Medical University Cancer CenterBeijing Shijitan Hospital
    • Department of Medical Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Peking University Cancer Hospital and Institute
    • Capital Medical University Cancer CenterBeijing Shijitan Hospital
  • Jiezhun Gu
    • Duke Clinical Research InstituteDuke University Medical Center
    • Department of SurgeryDuke University Medical Center
Original Article

DOI: 10.1007/s00262-013-1424-8

Cite this article as:
Song, G., Wang, X., Jia, J. et al. Cancer Immunol Immunother (2013) 62: 1123. doi:10.1007/s00262-013-1424-8

Abstract

Purpose

Suppression of cellular immunity resulting from tumorigenesis and/or therapy might promote cancer cells’ growth, progression and invasion. Here, we explored whether T lymphocyte subtypes from peripheral blood of metastatic breast cancer (MBC) female patients could be used as alternative surrogate markers for cancer progress. Additionally, plasma levels of interleukin (IL)-2, IL-4, IL-6, IL-10, IFN-γ, and transforming growth factor-β1 were quantitated from MBC and healthy volunteers.

Experimental design

This study included 89 female MBC patients during the post-salvage chemotherapy follow-up and 50 age- and sex-matched healthy volunteers as control. The percentages of T lymphocyte subpopulations from peripheral blood and plasma levels of cytokines were measured.

Results

Both CD8+CD28 and CD4+CD25+ were elevated in MBC patients compared to the control cohort (P < 0.05). In contrast, CD3+ and CD8+CD28+cells were significantly lower in MBC patients (P < 0.0001, P = 0.045, respectively). MBC patients had elevated levels of immunosuppressive cytokines IL-6 and IL-10. Patients with elevated CD8+CD28 and CD4+CD25+ cells showed increased levels of IL-6, and only patients with elevated CD8+CD28 had decreased interferon-γ. Univariate analysis indicated increased CD3+CD4+ or CD8+CD28+correlated with prolonged progression-free survival (PFS), while elevated CD8+CD28associated with shorten PFS. The percent of CD8+CD28 T lymphocytes is an independent predictor for PFS through multivariate analysis.

Conclusions

This study suggests that progressive elevated levels of CD8+CD28 suppressor T lymphocytes represent a novel independent predictor of PFS during post-chemotherapy follow-up.

Keywords

Regulatory T lymphocytePeripheral bloodMetastatic breast cancerProgression-free survivalCytokine

Copyright information

© Springer-Verlag Berlin Heidelberg 2013