Cancer Immunology, Immunotherapy

, Volume 62, Issue 3, pp 553–561

Higher numbers of T-bet+ intratumoral lymphoid cells correlate with better survival in gastric cancer

  • Lu-jun Chen
  • Xiao Zheng
  • Yue-ping Shen
  • Yi-bei Zhu
  • Qing Li
  • Junjun Chen
  • Rui Xia
  • Shu-ming Zhou
  • Chang-ping Wu
  • Xue-guang Zhang
  • Bin-feng Lu
  • Jing-ting Jiang
Original article

DOI: 10.1007/s00262-012-1358-6

Cite this article as:
Chen, L., Zheng, X., Shen, Y. et al. Cancer Immunol Immunother (2013) 62: 553. doi:10.1007/s00262-012-1358-6

Abstract

In the present study, we studied the expression of T-bet, a key marker for type 1 immune responses, within the tumor microenvironment of gastric cancer, and analyzed its association with clinicopathological parameters. One hundred and fifty-two archival paraffin-embedded gastric tumor tissues were collected, and the expression of T-bet in these cancer tissue specimens was examined by immunohistochemistry. T-bet+ tumor-infiltrating lymphocytes (TILs) in some gastric cancer tissues were further characterized by flow cytometric analysis. The density of T-bet+ TILs in gastric cancer tissues in relation to patient’s clinicopathological parameters and postoperative prognosis has been analyzed. Herein, we have found significant increases in T-bet+ lymphocytes in tumor tissues as compared with normal stomach tissues, gastritis tissues or gastric polyp specimens. T-bet+ cells mainly consisted of CD4+, CD8+ and CD56+ TILs. In addition, lower numbers of T-bet+ TILs were associated with poor clinicopathological parameters such as invasion to muscular layer, larger tumor size and advanced cancer stages. Moreover, patients with higher numbers of T-bet+ TILs have longer disease-free survival and overall survival. Thus, our study supports the idea that tumor growth elicits spontaneous type 1 cellular immune responses and tumor progression is associated with suppression of antitumor immunity. T-bet expression within tumor can serve as a prognostic indicator for gastric cancer and a potential biomarker for immunotherapy.

Keywords

T-betTumor-infiltrating lymphocyteGastric cancerPrognosis

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Lu-jun Chen
    • 1
  • Xiao Zheng
    • 1
  • Yue-ping Shen
    • 2
  • Yi-bei Zhu
    • 3
  • Qing Li
    • 4
  • Junjun Chen
    • 1
  • Rui Xia
    • 3
  • Shu-ming Zhou
    • 5
  • Chang-ping Wu
    • 1
  • Xue-guang Zhang
    • 3
  • Bin-feng Lu
    • 6
  • Jing-ting Jiang
    • 1
  1. 1.Department of Tumor Biological TreatmentThe Third Affiliated Hospital of Soochow UniversityChangzhouChina
  2. 2.Department of MedicineSoochow UniversitySuzhouChina
  3. 3.Key Laboratory of Clinical Immunology of Jiangsu Province, Institute of BiotechnologySoochow UniversitySuzhouChina
  4. 4.Department of PathologyThe Third Affiliated Hospital of Soochow UniversityChangzhouChina
  5. 5.Department of Tumor Biological TreatmentYixing Tumor HospitalWuxiChina
  6. 6.Department of ImmunologyUniversity of PittsburghPittsburghUSA