Cancer Immunology, Immunotherapy

, Volume 62, Issue 3, pp 553–561

Higher numbers of T-bet+ intratumoral lymphoid cells correlate with better survival in gastric cancer

Authors

  • Lu-jun Chen
    • Department of Tumor Biological TreatmentThe Third Affiliated Hospital of Soochow University
  • Xiao Zheng
    • Department of Tumor Biological TreatmentThe Third Affiliated Hospital of Soochow University
  • Yue-ping Shen
    • Department of MedicineSoochow University
  • Yi-bei Zhu
    • Key Laboratory of Clinical Immunology of Jiangsu Province, Institute of BiotechnologySoochow University
  • Qing Li
    • Department of PathologyThe Third Affiliated Hospital of Soochow University
  • Junjun Chen
    • Department of Tumor Biological TreatmentThe Third Affiliated Hospital of Soochow University
  • Rui Xia
    • Key Laboratory of Clinical Immunology of Jiangsu Province, Institute of BiotechnologySoochow University
  • Shu-ming Zhou
    • Department of Tumor Biological TreatmentYixing Tumor Hospital
  • Chang-ping Wu
    • Department of Tumor Biological TreatmentThe Third Affiliated Hospital of Soochow University
  • Xue-guang Zhang
    • Key Laboratory of Clinical Immunology of Jiangsu Province, Institute of BiotechnologySoochow University
    • Department of ImmunologyUniversity of Pittsburgh
    • Department of Tumor Biological TreatmentThe Third Affiliated Hospital of Soochow University
Original article

DOI: 10.1007/s00262-012-1358-6

Cite this article as:
Chen, L., Zheng, X., Shen, Y. et al. Cancer Immunol Immunother (2013) 62: 553. doi:10.1007/s00262-012-1358-6

Abstract

In the present study, we studied the expression of T-bet, a key marker for type 1 immune responses, within the tumor microenvironment of gastric cancer, and analyzed its association with clinicopathological parameters. One hundred and fifty-two archival paraffin-embedded gastric tumor tissues were collected, and the expression of T-bet in these cancer tissue specimens was examined by immunohistochemistry. T-bet+ tumor-infiltrating lymphocytes (TILs) in some gastric cancer tissues were further characterized by flow cytometric analysis. The density of T-bet+ TILs in gastric cancer tissues in relation to patient’s clinicopathological parameters and postoperative prognosis has been analyzed. Herein, we have found significant increases in T-bet+ lymphocytes in tumor tissues as compared with normal stomach tissues, gastritis tissues or gastric polyp specimens. T-bet+ cells mainly consisted of CD4+, CD8+ and CD56+ TILs. In addition, lower numbers of T-bet+ TILs were associated with poor clinicopathological parameters such as invasion to muscular layer, larger tumor size and advanced cancer stages. Moreover, patients with higher numbers of T-bet+ TILs have longer disease-free survival and overall survival. Thus, our study supports the idea that tumor growth elicits spontaneous type 1 cellular immune responses and tumor progression is associated with suppression of antitumor immunity. T-bet expression within tumor can serve as a prognostic indicator for gastric cancer and a potential biomarker for immunotherapy.

Keywords

T-betTumor-infiltrating lymphocyteGastric cancerPrognosis

Copyright information

© Springer-Verlag Berlin Heidelberg 2012