Cancer Immunology, Immunotherapy

, Volume 61, Issue 2, pp 215–221

Hypericin-based photodynamic therapy induces surface exposure of damage-associated molecular patterns like HSP70 and calreticulin

  • Abhishek D. Garg
  • Dmitri V. Krysko
  • Peter Vandenabeele
  • Patrizia Agostinis
Short communication

DOI: 10.1007/s00262-011-1184-2

Cite this article as:
Garg, A.D., Krysko, D.V., Vandenabeele, P. et al. Cancer Immunol Immunother (2012) 61: 215. doi:10.1007/s00262-011-1184-2

Abstract

Surface-exposed HSP70 and calreticulin are damage-associated molecular patterns (DAMPs) crucially involved in modulating the success of cancer therapy. Photodynamic therapy (PDT) involves the administration of a photosensitising (PTS) agent followed by visible light-irradiation. The reactive oxygen species that are thus generated directly kill tumours by damaging their microvasculature and inducing a local inflammatory reaction. PDT with the PTS photofrin is associated with DAMPs exposure, but the same is not true for other PTSs. Here, we show that when cancer cells are treated with hypericin-based PDT (Hyp-PDT), they surface-expose both HSP70 and calreticulin (CRT). Induction of CRT exposure was not accompanied by co-exposure of ERp57, but this did not compromise the ability of the exposed CRT to regulate the phagocytosis of Hyp-PDT-treated cancer cells by dendritic cells. Interestingly, we found that Hyp-PDT-induced CRT exposure (in contrast to anthracycline-induced CRT exposure) was independent of the presence of ERp57. Our results indicate that Hyp-PDT is a potential anti-cancer immunogenic modality.

Keywords

Calreticulin HSP70 Photodynamic therapy Hypericin Cancer DAMPs 

Abbreviations

CNTR

Control(s)

DAMP(s)

Damage-associated molecular pattern(s)

DC

Dendritic cells

Ecto-CRT

Surface externalised-calreticulin

Ecto-HSP70

Surface externalised-Heat shock protein 70

ER

Endoplasmic reticulum

HSP

Heat shock proteins

Hyp-PDT

Hypericin-based photodynamic therapy

MTX

Mitoxantrone

PDT

Photodynamic therapy

PTS(s)

Photosensitiser(s)

Supplementary material

262_2011_1184_MOESM1_ESM.pdf (158 kb)
Supplementary material 1 (PDF 157 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Abhishek D. Garg
    • 1
  • Dmitri V. Krysko
    • 2
    • 3
  • Peter Vandenabeele
    • 2
    • 3
  • Patrizia Agostinis
    • 1
  1. 1.Cell Death Research and Therapy Unit, Department of Molecular Cell Biology, Faculty of MedicineCatholic University of LeuvenLeuvenBelgium
  2. 2.Molecular Signaling and Cell Death Unit, Department for Molecular Biomedical ResearchVIBGhentBelgium
  3. 3.Department of Biomedical Molecular BiologyGhent UniversityGhentBelgium

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