Cancer Immunology, Immunotherapy

, Volume 61, Issue 1, pp 101–108

Upregulation of B7-H1 expression is associated with macrophage infiltration in hepatocellular carcinomas

  • Jie Chen
  • Guosheng Li
  • Hong Meng
  • Yuchen Fan
  • Yonghong Song
  • Shurong Wang
  • Faliang Zhu
  • Chun Guo
  • Lining Zhang
  • Yongyu Shi
Original article

DOI: 10.1007/s00262-011-1094-3

Cite this article as:
Chen, J., Li, G., Meng, H. et al. Cancer Immunol Immunother (2012) 61: 101. doi:10.1007/s00262-011-1094-3

Abstract

The overexpression of B7-H1 in hepatocellular carcinoma (HCC) mediates HCC immune escape and obstructs the immunotherapy based on tumor-specific CD8+ T cells. Tumor-associated macrophages (TAM) are a major component of cancer-related inflammation and play a central role in tumor promotion. To classify the mechanism underlying the overexpression of B7-H1 in HCC, we examined B7-H1 expression and TAM infiltration in 63 cases of human HCC samples using immunohistochemistry method and found that B7-H1 overexpression was associated with TAM infiltration in HCC tissues. Furthermore, B7-H1 expression was upregulated at both mRNA level and protein level in HCC cells (BEL-7402 and SMMC-7721) cocultured with macrophages in a transwell system. The upregulation of B7-H1 expression induced by macrophage was inhibited by blocking NF-κB or STAT3 signal pathways. These results suggest that overexpression of B7-H1 in HCC may be induced by inflammatory microenvironment involving macrophages and imply that anti-inflammation therapy might be preventive for immune escape and assistant for immunotherapy of HCC.

Keywords

B7-H1CD274Hepatocellular carcinomaPD-L1Tumor-associated macrophages

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Jie Chen
    • 1
  • Guosheng Li
    • 1
  • Hong Meng
    • 2
  • Yuchen Fan
    • 3
  • Yonghong Song
    • 4
  • Shurong Wang
    • 4
  • Faliang Zhu
    • 1
  • Chun Guo
    • 1
  • Lining Zhang
    • 1
  • Yongyu Shi
    • 1
  1. 1.Institute of Immunology, School of MedicineShandong UniversityJinanChina
  2. 2.Institute of Basic MedicineShandong Academy of Medical ScienceShandongChina
  3. 3.Department of HepatologyQilu Hospital of Shandong UniversityShandongChina
  4. 4.Shandong Blood CenterShandongChina